Inhibition of antiassociation activity of translation initiation factor 3 by paromomycin
Inhibition of antiassociation activity of translation initiation factor 3 by paromomycin
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DOI：
10.1128/aac.01096-06
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发表时间：
2007-01-01
影响因子：
4.9
通讯作者：
Kaji, Akira
中科院分区：
文献类型：
--
作者：
Hirokawa, Go;Kaji, Hideko;Kaji, Akira
研究了巴龙霉素对核糖体亚基相互作用的影响。巴龙霉素抑制起始因子3（IF3）的抗结合活性。此外，核糖体亚基相关联，形成70 S核糖体巴龙霉素，即使在1 MM Mg 2+的存在下。巴龙霉素不抑制IF3与30S核糖体亚基的结合。另一方面，与30S亚基结合的IF3通过巴龙霉素诱导的亚基缔合（70S形成）而被排出。这些结果表明巴龙霉素对70S核糖体的稳定性可能是其对易位和核糖体再循环的抑制作用的部分原因。
The effect of paromomycin on the interaction of ribosomal subunits was studied. Paromomycin inhibited the antiassociation activity of initiation factor 3 (IF3). Furthermore, ribosomal subunits were associated to form 70S ribosomes by paromomycin even in the presence of 1 MM Mg2+. Paromomycin did not inhibit the binding of IF3 to the 30S ribosomal subunits. On the other hand, IF3 bound to the 30S subunits was expelled by paromomycin-induced subunit association (70S formation). These results indicate that the stabilization of 70S ribosomes by paromomycin may in part be responsible for its inhibitory effects on translocation and ribosome recycling.