Production of polyclonal antisera.
Production of polyclonal antisera.
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DOI：
10.1002/0471142735.im0204s13
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发表时间：
2001-05-01
影响因子：
--
通讯作者：
Paterson, Yvonne
中科院分区：
文献类型：
--
作者：
Cooper, Helen M;Paterson, Yvonne
尽管单抗技术的发展使抗体的特异性、一致性和数量发生了革命性的变化，但在许多情况下，多克隆抗体比单抗更可取。生产多克隆抗血清比生产单抗花费更少的时间和精力，需要相对简单和容易获得的设备，并产生可用于免疫沉淀、免疫印迹和酶联免疫吸附分析(ELISA)的试剂。本单元中的基本方案和替代方案描述了针对兔、大鼠、小鼠和仓鼠的蛋白质抗原的多克隆抗血清的生产。支持协议提出了一种从血液中制备血清的方法。
Although the advances offered by the development of monoclonal antibody techniques have revolutionized the specificity, uniformity, and quantity of antibodies, there remain many circumstances in which polyclonal antibodies are more desirable than monoclonal antibodies. Production of polyclonal antisera takes less time and effort than production of monoclonal antibodies, requires relatively simple and readily available equipment, and produces reagents that can be used for immunoprecipitation, immunoblotting, and enzyme-linked immunosorbent assays (ELISAs). The Basic Protocol and Alternate Protocol in this unit describe the production of polyclonal antisera specific for protein antigens in rabbits, rats, mice, and hamsters. The Support Protocol presents a method for preparing serum from blood.