Lasting effects of an impairment of Th1-like immune response in γ-irradiated mice: A resemblance between irradiated mice and aged mice
Lasting effects of an impairment of Th1-like immune response in γ-irradiated mice: A resemblance between irradiated mice and aged mice
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DOI：
10.1016/j.cellimm.2010.10.004
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发表时间：
2011-01-01
影响因子：
4.3
通讯作者：
Jo, Sung-Kee
中科院分区：
文献类型：
--
作者：
Park, Hae-Ran;Jo, Sung-Kee
虽然电离辐射的几个慢性效应之一是衰老，但没有关于辐射诱导的免疫衰老的实验数据。最有趣的衰老变化是辅助性T（Th）1/Th 2失衡。本文研究了电离辐射对小鼠免疫功能的慢性影响，并与老年小鼠进行了比较。2个月大的小鼠接受5戈伊的全身照射。在照射后6个月，我们比较了照射小鼠与同龄正常小鼠和老年小鼠的免疫功能。γ-干扰素（IFN）和抗原特异性免疫球蛋白（IG）G2 a水平低于同龄正常小鼠，与老年正常小鼠相似。与此相反，白细胞介素（IL）-4和IL-5和抗原特异性IgG 1水平在照射小鼠与同龄正常小鼠相比，增加。接下来，我们研究了IL-12 p70、IL-12受体和IL-18受体在辐射和老年小鼠中的低表达。同时，自然杀伤细胞活性的降低在受照射小鼠中加剧，显示出低于老年小鼠的值。有趣的是，在受辐射的小鼠中，自然杀伤（NK）细胞的绝对数量和百分比都大大减少。但老年小鼠Th细胞和细胞毒性T（Tc）细胞绝对数明显下降。总之，5戈伊全身照射引起的免疫失衡会长期存在，导致与衰老相似的结果。我们的研究结果表明，下调的Th 1样免疫反应显示在老年小鼠迅速发生通过电离辐射暴露。(C)2010年爱思唯尔公司All rights reserved.
Although one of the several chronic effects of ionizing radiation is aging, there are no experimental data on radiation-induced immunological aging. The most interesting change in aging was a helper T (Th) 1/Th2 imbalance. We investigated chronic effect on immune responses after ionizing radiation and its effects in irradiated mice were compared with those of aged mice. The 2-month-old mice received a whole-body irradiation of 5 Gy. At 6 months after irradiation, we compared the immune functions of the irradiated mice with those of normal mice of the same age and with those of older. Interferon (IFN)-gamma and antigen-specific immunoglobulin (Ig)G2a level were lower in the irradiated mice than in normal mice of same age, showing similar levels to those of old normal mice. In contrast, interleukin (IL)-4 and IL-5 and antigen-specific IgG1 level were increased in irradiated mice when compared with the same aged-normal mice. Next, we investigated the low expression of IL-12p70, IL-12 receptors and IL-18 receptors in irradiated and old mice. Also, the decrease of natural killer cell activity was intensified in the irradiated mice, showing lower than values to those of old mice. Interestingly, in irradiated mice, the absolute numbers and the percentages of natural killer (NK) cells was extremely decreased. But the absolute numbers of Th cells and cytotoxic T (Tc) cells in old mice were significantly decreased. In conclusion, an immunological imbalance by the whole-body irradiation of 5 Gy induces to persist in the long term, resulting in the similar results with aging. Our results suggest that the downregulation of the Th1-like immune response shown in old mice rapidly occurred through exposure of ionizing radiation. (C) 2010 Elsevier Inc. All rights reserved.