BREATHLESS, A DROSOPHILA FGF RECEPTOR HOMOLOG, IS ESSENTIAL FOR MIGRATION OF TRACHEAL AND SPECIFIC MIDLINE GLIAL-CELLS
BREATHLESS, A DROSOPHILA FGF RECEPTOR HOMOLOG, IS ESSENTIAL FOR MIGRATION OF TRACHEAL AND SPECIFIC MIDLINE GLIAL-CELLS
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DOI：
10.1101/gad.6.9.1668
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发表时间：
1992-09-01
影响因子：
10.5
通讯作者：
SHILO, BZ
中科院分区：
文献类型：
--
作者：
KLAMBT, C;GLAZER, L;SHILO, BZ
分离出了成纤维细胞生长因子（FGF）受体的果蝇同源物，并对其结构进行了表征。在对插入该基因上游的标记P元件进行EMS诱变或不精确切除后，分离出了该基因座的一系列表型突变。这些突变体在受体表达的两个胚胎组织——气管系统和中线中表现出缺陷。在严重的突变体中，气管细胞无法迁移，仍留在气管凹坑内。亚效等位基因表现出所有气管分支的部分迁移；因此，该基因座被命名为“气喘吁吁”（btl）。在突变胚胎的中线中，后部的一对中线神经胶质细胞开始向前迁移，但无法到达后连合。细胞迁移异常似乎是这两个不同组织中btl缺陷的共同特征。在亚效突变体中，细胞表现出部分迁移，但遵循正常路径，这表明该受体的存在对于迁移细胞识别外部导向信号的能力至关重要。
A Drosophila homolog of the fibroblast growth factor (FGF) receptor was isolated and structurally characterized. After EMS mutagenesis or imprecise excisions of marked P elements inserted upstream to the gene, a phenotypic series of mutations in the locus was isolated. The mutants exhibit defects in the two embryonic tissues in which the receptor is expressed: the tracheal system and the midline. The tracheal cells fail to migrate in severe mutants and remain within the tracheal pits. Hypomorphic alleles exhibit partial migration of all tracheal branches; thus, the locus was termed breathless (btl). In the midline of the mutant embryos, the posterior pair of midline glial cells begins to migrate anteriorly, but fails to reach the posterior commissure. Abnormalities in cell migration appear to be a common denominator for the btl defects in these two disparate tissues. In hypomorphic mutants the cells exhibit partial migration but follow the normal tracts, suggesting that the presence of this receptor is essential for the ability of the migrating cells to recognize external guiding cues.