Green fluorescent protein-transgenic rat: A tool for organ transplantation research
Green fluorescent protein-transgenic rat: A tool for organ transplantation research
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DOI：
10.1006/bbrc.2001.5452
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发表时间：
2001-08-31
影响因子：
3.1
通讯作者：
Kobayashi, E
中科院分区：
文献类型：
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作者：
Hakamata, Y;Tahara, K;Kobayashi, E
本研究的目的是评估绿色荧光蛋白（GFP）转基因大鼠作为器官移植研究工具的用途。 GFP 基因构建体被设计为广泛表达。通过流式细胞术，从GFP转基因大鼠的骨髓、脾脏和外周血中获得的细胞分别由77%、91%和75%的GFP阳性细胞组成。为了检查器官移植后 GFP 阳性细胞的细胞迁移，还从 GFP 转基因大鼠向 LEW (RT1(1)) 大鼠进行了胰腺移植（伴或不伴脾移植）、心脏移植（伴或不伴肺移植）、辅助肝和小肠移植，疗程为 2 周，给予 0.64 mg/kg 他克莫司。器官移植后的完全同种异体 LEW 大鼠中检测到 GFP 阳性供体细胞。这些结果表明，GFP转基因大鼠是器官移植研究的有用工具，例如器官移植后无需供体细胞染色的细胞迁移研究。 (C) 2001 年学术出版社。
The purpose of this study is to evaluate green fluorescent protein (GFP) transgenic rats for use as a tool for organ transplantation research. The GFP gene construct was designed to express ubiquitously. By flow cytometry, the cells obtained from the bone marrow, spleen, and peripheral blood of the GFP transgenic rats consisted of 77, 91, and 75% GFP-positive cells, respectively. To examine cell migration of GFP-positive cells after organ transplantation, pancreas graft with or without spleen transplantation, heart graft with or without lung transplantation, auxiliary liver and small bowel transplantation were also performed from GFP transgenic rat to LEW (RT1(1)) rats under a 2-week course of 0.64 mg/kg tacrolimus administration. GFP-positive donor cells were detected in the fully allogenic LEW rats after organ transplantation. These results showed that GFP transgenic rat is a useful tool for organ transplantation research such as cell migration study after organ transplantation without donor cell staining. (C) 2001 Academic Press.