Cis-acting mutation and duplication: History of molecular evolution in a P450 haplotype responsible for insecticide resistance in Culex quinquefasciatus
Cis-acting mutation and duplication: History of molecular evolution in a P450 haplotype responsible for insecticide resistance in Culex quinquefasciatus
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DOI：
10.1016/j.ibmb.2011.04.002
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发表时间：
2011-07-01
影响因子：
3.8
通讯作者：
Tomita, Takashi
中科院分区：
文献类型：
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作者：
Itokawa, Kentaro;Komagata, Osamu;Tomita, Takashi
致倦库蚊（Culex quinquefasciatus，JPal-per）细胞色素P450基因Cyp 9 m10在拟除虫菊酯抗性品系中的表达量增加了200倍以上。该菌株的单倍型含有两个拷贝的Cyp 9 m10，由最近的串联重复产生。在这项研究中，我们发现并分离出一个Cyp 9 m10单倍型密切相关的复制Cyp 9 m10单倍型从JHB，一个菌株用于最近的基因组计划，这种蚊子物种。分离的单倍型（JHB-NIID-B单倍型）与JPal-per株共享编码区上游的转座因子的相同插入，但不重复。JHB-NIID-B单倍型被认为在复制事件之前从JPal-per谱系中分离出来。Cyp 9 m10在具有JHB-NIID-B单倍型的幼虫中中度过表达。JHB-NIID-B和JPal-per单倍型中的过度表达以相似的模式发育调节，表明两种单倍型共享负责过度表达的共同顺式作用突变。分离的中度过表达的单倍型赋予抗性，然而，其效力相对较小。我们假设，第一顺式作用突变修改的结果，随后的重复在JPal每谱系赋予更强的表型效应比，如果它发生在第一顺式作用突变。(C)2011爱思唯尔有限公司保留所有权利。
A cytochrome P450 gene, Cyp9m10, is more than 200-fold oyerexpressed in a pyrethroid resistant strain of Culex quinquefasciatus,JPal-per. The haplotype of this strain contains two copies of Cyp9m10 resulted from recent tandem duplication. In this study, we discovered and isolated a Cyp9m 10 haplotype closely related to this duplicated Cyp9m10 haplotype from JHB, a strain used for the recent genome project for this mosquito species. The isolated haplotype (JHB-NIID-B haplotype) shared the same insertion of a transposable element upstream of the coding region with JPal-per strain but not duplicated. The JHB-NIID-B haplotype was considered to have diverged from the JPal-per lineage just before the duplication event. Cyp9m10 was moderately overexpressed in larvae with the JHB-NIID-B haplotype. The overexpressions in JHB-NIID-B and JPal-per haplotypes were developmentally regulated in similar pattern indicating both haplotypes share a common cis-acting mutation responsible for the overexpressions. The isolated moderately overexpressed haplotype conferred resistance, however, its efficacy was relatively small. We hypothesized that the first cis-acting mutation modified the consequence of the subsequent duplication in JPal-per lineage to confer stronger phenotypic effect than that if it occurred before the first cis-acting mutation. (C) 2011 Elsevier Ltd. All rights reserved.