The genome of turkey herpesvirus
The genome of turkey herpesvirus
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DOI：
10.1128/jvi.75.2.971-978.2001
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发表时间：
2001-01-01
影响因子：
5.4
通讯作者：
Kutish, GF
中科院分区：
文献类型：
--
作者：
Afonso, CL;Tulman, ER;Kutish, GF
在这里，我们提出了第一个完整的基因组序列的马立克氏病病毒血清3型(MDV3)，也被称为火鸡疱疹病毒(HVT)。该病毒基因组全长159,160个碱基，编码99个可能的蛋白质，在基因组组织和基因含量上与甲型疱疹病毒相似，在独特的长(UL)和短(US)基因组区域内与MDV1和MDV2非常相似，同源基因具有高度的共线性，它们的蛋白质具有高度的氨基酸同源性。在UL区，HVT包含57个在1型单纯疱疹病毒(HSV-1)中发现的同源物基因，6个仅在MDV中发现的同源物基因，以及两个耳鼻喉科独有的基因(HVT068和HVT070基因)。由于缺乏MDV093(SORF4)同源物以及UL/短重复序列(RS)边界的差异，HVT US区比MDV1(MD5株)短2.2kb。HVT缺乏MDV087的同源物，MDV087是一种编码在MDV1(MD5)的UL/RS边界的蛋白质，它在RS中含有MDV096(糖蛋白E)的两个同源物。Hvt-RS比MDV1长1,039个碱基，除与MDV1基因同源外，其基因含量与MDV1不同。在RS中发现了6个独特的基因，其中包括抗凋亡基因Bcl-2的同源物。这是甲型疱疹病毒中首次报道的Bcl2同源基因。HVT长重复序列(RL)比MDV1短7,407个碱基，不包含MDV1基因的同源基因，这些基因的功能涉及毒力、致癌性和免疫逃避。HVT缺乏MDV1癌蛋白MEQ、CXC趋化因子、致癌相关磷蛋白pp24的同源基因，以及磷蛋白pp38的保守结构域。这些在RS和RL区域及其附近的显著基因组差异可能解释了非致病性HVT和高致病性MDV1在宿主范围、毒力和致瘤性方面的差异。
Here we present the first complete genomic sequence of Marek's disease virus serotype 3 (MDV3), also known as turkey herpesvirus (HVT). The 159,160-bp genome encodes an estimated 99 putative proteins and resembles alphaherpesviruses in genomic organization and gene content, HVT is very similar to MDV1 and MDV2 within the unique long (UL) and unique short (US) genomic regions, where homologous genes share a high degree of colinearity and their proteins share a high level of amino acid identity. Within the UL region, HVT contains 57 genes with homologues found in herpes simplex virus type 1 (HSV-1), six genes with homologues found only in MDV, and two genes (HVT068 and HVT070 genes) which are unique to ENT. The HVT US region is 2.2 kb shorter than that of MDV1 (Md5 strain) due to the absence of an MDV093 (SORF4) homologue and to differences at the UL/short repeat (RS) boundary. HVT lacks a homologue of MDV087, a protein encoded at the UL/RS boundary of MDV1 (Md5), and it contains two homologues of MDV096 (glycoprotein E) in the RS. HVT RS are 1,039 bp longer than those in MDV1, and with the exception of an ICP 1 gene homologue, the gene content is different from that of MDV1. Six unique genes, including a homologue of the antiapoptotic gene Bcl-2, are found in the RS. This is the first reported Bcl-2 homologue in an alphaherpesvirus. HVT long repeats (RL) are 7,407 bp shorter than those in MDV1 and do not contain homologues of MDV1 genes with functions involving virulence, oncogenicity, and immune evasion. HVT lacks homologues of MDV1 oncoprotein MEQ, CxC chemokine, oncogenicity-associated phosphoprotein pp24, and conserved domains of phosphoprotein pp38. These significant genomic differences in and adjacent to RS and RL regions likely account for the differences in host range, virulence, and oncogenicity between nonpathogenic HVT and highly pathogenic MDV1.