Bioactivity of Scytonema hofmanni (Cyanobacteria) in Lilium alexandrae in vitro propagation
Bioactivity of Scytonema hofmanni (Cyanobacteria) in Lilium alexandrae in vitro propagation
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DOI：
10.2225/vol9-issue3-fulltext-30
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发表时间：
2006-06-01
影响因子：
2.7
通讯作者：
Stella, Ana María
中科院分区：
文献类型：
--
作者：
Zaccaro, María Cristina;Kato, Adriana;Stella, Ana María
蓝细菌产生生物活性化合物，包括植物生长调节剂。萘乙酸（NAA）是一种人工合成的植物生长调节剂，用于微繁殖。本工作的目的是评估形态发生和抗氧化作用所产生的内和胞外物质从Scytonema hofmanni（蓝细菌）在体外增殖的百合亚历山大，并比较他们所产生的NAA。内和胞外蓝藻产品增加a）小鳞茎生产达到83%和78%的NAA效果，分别; B）小鳞茎直径相比，NAA;和c）小鳞茎的生存，由于促进抗氧化活性测量为过氧化氢酶，抗坏血酸过氧化物酶，谷胱甘肽还原酶活性。蓝藻物质促进鳞茎再生，延缓鳞茎衰老。它们不仅在再生阶段，而且在离体培养的有活力的小鳞茎的储存过程中，都可以取代对操作者有危险的NAA。
Cyanobacteria produces bioactive compounds including plant growth regulators. Naphthalene acetic acid (NAA), a toxic substance, is a synthetic plant regulator used in micropropagation. The aim of this work was to evaluate morphogenetic and antioxidant effects produced by intra and extracellular substances from Scytonema hofmanni (Cyanobacteria) during the multiplication in vitro of Lilium alexandrae and to compare them to those produced by NAA. Intra and extracellular cyanobacterial products increased a) bulblets production reaching 83% and 78% of NAA effect, respectively; b) the bulblet diameter compared to NAA; and c) the bulblet survival due to the promotion of antioxidant activity measured as catalase, ascorbate peroxidase, and glutathione reductase activity. The cyanobacterial substances stimulated regeneration and delayed bulblet senescence. They could replace NAA, dangerous for the operator, not only during the regeneration phase but also during the storage of the viable bulblets cultivated in vitro.