Copper-induced growth inhibition, oxidative stress and ultrastructural alterations in freshly grown water lettuce (Pistia stratiotes L.).
Copper-induced growth inhibition, oxidative stress and ultrastructural alterations in freshly grown water lettuce (Pistia stratiotes L.).
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DOI：
10.1016/j.crvi.2009.03.001
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发表时间：
2009-07
影响因子：
2
通讯作者：
R K Upadhyay;S. Panda
中科院分区：
文献类型：
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作者：
R K Upadhyay;S. Panda
研究了不同浓度铜对水浮莲生长、超微结构和某些生化指标的影响。在控制条件下，在含有增加的硫酸铜浓度范围为0至100 μM的营养液中进行了研究。铜处理12、18和24 h对根和叶干生物量均产生抑制作用。根和叶的原子吸收光谱分析表明，铜的积累随着金属处理浓度和时间的增加而增加。可见铜导致根和叶细胞中过氧化氢和超氧自由基的产生增加，在处理24 h后显示出显著变化。总蛋白质和光合色素含量显著降低。抗氧化酶，即，过氧化物酶（POX，EC 1.11. 1.7）、过氧化氢酶（CAT，EC 1.11. 1.6)和超氧化物歧化酶（SOD，EC 1.15. 1.1)随着脂质过氧化程度的增加，抗坏血酸和谷胱甘肽水平呈上升趋势。这些酶的快速诱导是有用的早期和敏感的重金属毒性指标。结果表明，高浓度Cu胁迫对水生莴苣的生理生化产生显著影响，诱导氧化胁迫，表现为膜脂过氧化反应的启动，抑制生长，导致主要抗氧化系统的解体。
The effects of increasing concentrations of copper on the growth, ultra-structure and on certain biochemical parameters of water lettuce (Pistia stratiotes L.) were investigated under controlled conditions in the nutrient solutions containing increased copper sulfate concentrations ranging from 0 to 100 μM. Copper treatment for 12, 18 or 24 h resulted in inhibition of roots and leaves dry biomass. Atomic absorption spectrometry analysis of roots and leaves showed that copper accumulation increased with increase in concentration and duration of metal treatment. It is seen that copper resulted in increased production of hydrogen peroxide and superoxide radical in both roots and leave cells, showed a significant change after 24 h of treatment. Also, the significant decrease in the contents of total protein and photosynthetic pigments was observed. The antioxidant enzymes, viz., peroxidase (POX, EC 1.11. 1.7), catalase (CAT, EC 1.11. 1.6) and superoxide dismutase (SOD, EC 1.15. 1.1) showed significant variation with the increase in lipid peroxidation. Increasing trends was observed in levels of ascorbate and glutathione. The rapid inducibility of some of these enzymes are useful early and sensitive indicators of heavy metal toxicity. The results demonstrated that exposure to elevated concentration of Cu had a remarkable effect on the biochemistry and physiology, induced oxidative stress in water lettuce characterized by the initiation of lipid peroxidation that inhibited growth and disintegration of major antioxidant systems.