The role of craniofacial growth in leptin deficient (ob/ob) mice.
The role of craniofacial growth in leptin deficient (ob/ob) mice.
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DOI：
10.1034/j.1600-0544.2003.00260.x
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发表时间：
2003-11-01
影响因子：
3.1
通讯作者：
Maki, K
中科院分区：
文献类型：
--
作者：
Yagasaki, Y;Yamaguchi, T;Maki, K
目的：利用遗传性肥胖模型 ob/ob 小鼠阐明瘦素对颌面部形态生长的作用，并检查小鼠髁头软骨中瘦素受体基因表达的存在。 设计：对 10 只 C57BL/6J（瘦）和 10 只 C57BL/6J-ob (ob/ob) 小鼠（瘦素）每天腹腔注射一次瘦素。出生后第 5 周至第 11 周期间，对 10 只瘦小鼠和 10 只 ob/ob 小鼠（PBS 给药组）注射磷酸盐缓冲盐水 (PBS)。第11周测量脂肪量、去脂体量、体脂率、颈髁宽度，第5、8、11周进行X线头影测量分析。此外，立体切除C57BL/6J小鼠髁头软骨，提取总RNA，对瘦素受体基因进行RT-PCR法。结果：瘦素产生不足的ob/ob小鼠的体脂量高于瘦素小鼠，且颌面部形态各测量项目均存在显着差异。通过给予瘦素，观察到 ob/ob 小鼠骨长度的恢复。此外，还证实了髁头软骨中瘦素受体基因的表达。结论：外源性瘦素给药导致颅面尺寸显着增加；瘦素受体在下颌髁软骨中表达。这些结果表明瘦素在颅面生长和形态中发挥重要作用。我们推测瘦素对骨和软骨的直接外周作用与这一作用密切相关。
OBJECTIVES: To elucidate the role of leptin on maxillo-facial morphological growth using hereditary obesity model ob/ob mice, and to examine the presence of the leptin receptor gene expression in the mouse condylar head cartilage.DESIGN: Leptin was intraperitoneally administered once a day in 10 C57BL/6J (lean) and 10 C57BL/6J-ob (ob/ob) mice (leptin administration group), and phosphate-buffered saline (PBS) in 10 lean and 10 ob/ob mice (PBS administration group), between the fifth and 11th week after birth. The amount of fat, the body amount without fat, the rate of body fat, and the width of the condylar cervical area were measured during the 11th week, and roentgenographic cephalometric analysis was performed at the fifth, eighth, and 11th week. Furthermore, the condylar head cartilage in C57BL/6J mice was stereoscopically excised to extract total RNA, and RT-PCR method was performed regarding the leptin receptor gene.RESULTS: The body fat amount in ob/ob mice with leptin production insufficiency was greater than that in lean mice, and significant differences were noted in every measurement item regarding maxillo-facial morphology. Recovery of bone length was noted in ob/ob mice by administering leptin. Furthermore, the expression of the leptin receptor gene in the condylar head cartilage was confirmed.CONCLUSION: Exogenous leptin administration leads to significant increases in craniofacial dimensions; and leptin receptors are expressed in mandibular condylar cartilage. These results indicate an important role for leptin in craniofacial growth and morphology. We speculate that leptin's direct peripheral effect on bone and cartilage is closely involved in this role.