REPAIR OF PYRIMIDINE DIMERS IN RADIATION-SENSITIVE MUTANTS RAD3, RAD4, RAD6 AND RAD9 OF SACCHAROMYCES-CEREVISIAE
REPAIR OF PYRIMIDINE DIMERS IN RADIATION-SENSITIVE MUTANTS RAD3, RAD4, RAD6 AND RAD9 OF SACCHAROMYCES-CEREVISIAE
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DOI：
10.1016/0027-5107(77)90038-0
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发表时间：
1977-01-01
期刊：
影响因子：
--
通讯作者：
PRAKASH, L
中科院分区：
文献类型：
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作者：
PRAKASH, L
检测了4个辐射敏感突变株对紫外线诱导的嘧啶二聚体的清除能力。啤酒。从照射细胞的DNA的敏感性切口由[噬菌体] T4紫外线内切核酸酶或在藤黄微球菌的粗提取物中发现的内切核酸酶活性被用来测量DNA中的二聚体的存在。rad 3和rad 4突变体在二聚体切除中是有缺陷的;而rad 6和rad 9突变体在二聚体切除中是熟练的。
The ability to remove UV-induced pyrimidine dimers was examined in 4 radiation-sensitive mutants of S. cerevisiae. The susceptibility of DNA from irradiated cells to nicking by either the [bacteriophage] T4 UV-endonuclease or an endonuclease activity found in crude extracts of Micrococcus luteus was used to measure the presence of dimers in DNA. The rad3 and rad4 mutants are defective in dimer excision; whereas the rad6 and rad9 mutants are proficient in dimer excision.