Homologous up-regulation of the 1,25 (OH)2 vitamin D3 receptor in rats.
Homologous up-regulation of the 1,25 (OH)2 vitamin D3 receptor in rats.
复制标题
大鼠体内 1,25 (OH)2 维生素 D3 受体的同源上调。
DOI：
10.1016/0006-291x(86)91141-1
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发表时间：
1986
影响因子：
3.1
通讯作者：
Feldman,D
中科院分区：
文献类型：
--
作者：
Costa,EM;Feldman,D
本研究调查了维生素 D 代谢物在体内调节 1,25(OH)2D3 受体的能力。维生素 D 缺乏的大鼠用 1,25(OH)2D3 或媒介物治疗 1-5 天。在接受治疗的动物中，肾脏中的 1,25(OH)2D3 受体与对照水平相比显着增加。 2 剂激素后，特异性结合增加至对照的 220%，治疗 5 天后达到 336%。治疗 5 天后，肠道受体仅增加至对照水平的 130%。在维生素 D 充足的动物中，当通过交换 (TPCK) 测量内源占据位点时，对照组和治疗组之间的差异稍大。然而，仅在激素治疗 4 天后才观察到显着变化。数据表明 1,25(OH)2D3 受体在体内发生同源上调。肾脏和肠道反应的差异表明不同器官中上调的重要性不同。
This study investigates the ability of vitamin D-metabolites to regulate 1,25(OH)2D3receptors in vivo. Rats made vitamin D-deficient were treated with 1,25(OH)2D3or vehicle for 1–5 days. In treated animals, receptors for 1,25(OH)2D3in kidney increased dramatically compared with control levels. An increase in specific binding to 220% of control was seen after 2 doses of hormone, which reached to 336% after 5 days of treatment. Intestinal receptors increased to only 130% of control levels after 5 days of treatment. In vitamin D-replete animals, the difference between control and treated groups was slightly greater when endogenously occupied sites were measured by exchange (TPCK). However, significant changes were observed only after 4 days of hormone treatment. The data indicate that homologous up-regulation of the 1,25(OH)2D3receptor occurs in vivo. The difference in response in kidney and in intestine suggests differential importance of up-regulation in various organs.