AN AUTORADIOGRAPHIC INVESTIGATION OF TOOTH RENEWAL IN PURPLE SEA URCHIN (STRONGYLOCENTROTUS PURPURATUS)
AN AUTORADIOGRAPHIC INVESTIGATION OF TOOTH RENEWAL IN PURPLE SEA URCHIN (STRONGYLOCENTROTUS PURPURATUS)
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DOI：
10.1002/jez.1401580304
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发表时间：
1965-01-01
影响因子：
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通讯作者：
HOLLAND, ND
中科院分区：
文献类型：
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作者：
HOLLAND, ND
紫海胆牙齿中的DNA合成细胞用氚化胸苷标记，并用放射自显影法检测。一小时后，在牙齿的软的背口端的远轴外周层中有许多细胞被标记。这些细胞是牙齿再生新细胞的主要来源。随着时间的推移，标记的细胞在远轴周围层（和几代他们的后代）迁移作为一个区域的标记细胞。该区域在口腔方向上向牙齿的硬咬端迁移。迁移带前部的标记细胞在牙齿中占据横向位置，迁移带后部的标记细胞占据中间位置。在实验室中保存的紫海胆年轻成年标本的牙齿更新时间约为75天。
The DNA‐synthesizing cells in the tooth of the purple sea urchin were labeled with tritiated thymidine and detected with autoradiography. After one hour many cells were labeled in the abaxial peripheral layer of the soft, aboral end of the tooth. These cells were the chief source of new cells for tooth renewal. With time, the cells labeled in the abaxial peripheral layer (and several generations of their progeny) migrated as a zone of labeled cells. The zone migrated in an oral direction toward the hard, biting end of the tooth. The labeled cells in the leading part of the migrating zone occupied lateral positions in the tooth, and the labeled cells in the trailing part of the migrating zone occupied a median position. The time of tooth renewal in young adult specimens of the purple sea urchin maintained in the laboratory is approximately 75 days.