Immunoproteasome responds to injury in the retina and brain.
Immunoproteasome responds to injury in the retina and brain.
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DOI：
10.1111/j.1471-4159.2008.05345.x
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发表时间：
2008-07
影响因子：
4.7
通讯作者：
Gregerson DS
中科院分区：
文献类型：
--
作者：
Ferrington DA;Hussong SA;Roehrich H;Kapphahn RJ;Kavanaugh SM;Heuss ND;Gregerson DS
众所周知，免疫蛋白酶体产生MHC I类占用和细胞毒性T淋巴细胞（CTL）识别的肽。目前的研究集中在免疫蛋白酶体替代作用的证据上。应用免疫组织化学和western blotting分析正常情况下和CTL攻击神经胶质（脑）或神经元（视网膜）损伤/应激后视网膜和脑组织中免疫蛋白酶体亚基的表达。正常视网膜在胶质细胞、神经元和视网膜色素上皮中表达大量的免疫蛋白酶体。视网膜免疫蛋白酶体基础水平比脑高2倍；ctl诱导的视网膜损伤进一步上调免疫蛋白酶体的表达。免疫蛋白酶体在损伤脑组织中也出现上调，并与浦肯野细胞、小胶质细胞、星形胶质细胞和少突胶质细胞的表达相对应。这些结果表明视网膜的正常环境具有足够的挑战性，需要免疫蛋白酶体的持续表达。此外，免疫蛋白酶体在视网膜和脑损伤中的上调暗示了其在神经元保护和/或损伤修复中的作用。
It is well known that immunoproteasome generates peptides for MHC Class I occupancy and recognition by cytotoxic T lymphocytes (CTL). The present study focused on evidence for alternative roles for immunoproteasome. Retina and brain were analyzed for expression of immunoproteasome subunits using immunohistochemistry and western blotting under normal conditions and after injury/stress induced by CTL attack on glia (brain) or neurons (retina). Normal retina expressed substantial levels of immunoproteasome in glia, neurons, and retinal pigment epithelium. The basal level of immunoproteasome in retina was two-fold higher than in brain; CTL-induced retinal injury further up-regulated immunoproteasome expression. Immunoproteasome up-regulation was also observed in injured brain and corresponded with expression in Purkinje cells, microglia, astrocytes, and oligodendrocytes. These results suggest that the normal environment of the retina is sufficiently challenging to require on-going expression of immunoproteasome. Further, immunoproteasome up-regulation with retinal and brain injury implies a role in neuronal protection and/or repair of damage.
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影响因子：
3.7
作者：
Díaz-Hernández, M;Martín-Aparicio, E;Lucas, JJ
通讯作者：
Lucas, JJ
影响因子：
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作者：
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3.3
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通讯作者：
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64.8
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通讯作者：
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影响因子：
4.8
作者：
Chatterjee-Kishore, M;Kishore, R;Ferrone, S
通讯作者：
Ferrone, S