Autocrine and Paracrine Roles for ATP and Serotonin in Mouse Taste Buds
Autocrine and Paracrine Roles for ATP and Serotonin in Mouse Taste Buds
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DOI：
10.1523/jneurosci.2351-09.2009
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发表时间：
2009-11-04
影响因子：
5.3
通讯作者：
Roper, Stephen D.
中科院分区：
文献类型：
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作者：
Huang, Yijen A.;Dando, Robin;Roper, Stephen D.
受体（II型）味蕾细胞在味觉刺激过程中分泌ATP。反过来，ATP激活相邻的突触前（III型）细胞释放5-羟色胺（5-HT）和去甲肾上腺素（NE）。这些神经递质在味蕾中的作用尚未完全阐明。在这里，我们测试是否ATP或5-HT施加反馈到受体（II型）细胞在味觉刺激。我们以前的研究表明NE似乎不作用于邻近的味蕾细胞，或至少不作用于受体细胞。我们的数据表明，从突触前（III型）细胞释放的5-HT通过激活5-HT 1A受体，抑制味觉诱发的受体细胞中的Ca 2+动员，并减少ATP分泌，提供对受体细胞的负旁分泌反馈。研究结果还表明，ATP通过激活P2 Y1受体和增强ATP分泌对受体（II型）细胞施加正自分泌反馈。这些结果开始整理出嘌呤能和胺能递质如何在味蕾中发挥作用，以调节这些外周感觉器官中的味觉信号。
Receptor (type II) taste bud cells secrete ATP during taste stimulation. In turn, ATP activates adjacent presynaptic (type III) cells to release serotonin (5-hydroxytryptamine, or 5-HT) and norepinephrine (NE). The roles of these neurotransmitters in taste buds have not been fully elucidated. Here we tested whether ATP or 5-HT exert feedback onto receptor (type II) cells during taste stimulation. Our previous studies showed NE does not appear to act on adjacent taste bud cells, or at least on receptor cells. Our data show that 5-HT released from presynaptic (type III) cells provides negative paracrine feedback onto receptor cells by activating 5-HT1A receptors, inhibiting taste-evoked Ca2+ mobilization in receptor cells, and reducing ATP secretion. The findings also demonstrate that ATP exerts positive autocrine feedback onto receptor (type II) cells by activating P2Y1 receptors and enhancing ATP secretion. These results begin to sort out how purinergic and aminergic transmitters function within the taste bud to modulate gustatory signaling in these peripheral sensory organs.