An extensive class of small RNAs in Caenorhabditis elegans
An extensive class of small RNAs in Caenorhabditis elegans
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DOI：
10.1126/science.1065329
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发表时间：
2001-10-26
期刊：
影响因子：
56.9
通讯作者：
Ambros, V
中科院分区：
文献类型：
--
作者：
Lee, RC;Ambros, V
lin-4和let-7反义RNA是通过抑制靶mRNA的翻译来控制秀丽隐杆线虫发育事件的时间的时间调节因子。let-7 RNA在两侧对称动物中是保守的，这表明这类小RNA [microRNA（miRNAs）]在进化上是古老的。利用生物信息学和cDNA克隆技术，我们在C.优雅的这些基因中有几个表达小的转录本，这些转录本在C. elegans幼虫发育，其中三个在哺乳动物和/或昆虫中有明显的同源物。小的非编码RNA的miRNA类似乎是众多和多样的。
The lin-4 and let-7 antisense RNAs are temporal regulators that control the timing of developmental events in Caenorhabditis elegans by inhibiting translation of target mRNAs. let-7 RNA is conserved among bilaterian animals, suggesting that this class of small RNAs [microRNAs (miRNAs)] is evolutionarily ancient. Using bioinformatics and cDNA cloning, we found 15 new miRNA genes in C. elegans. Several of these genes express small transcripts that vary In abundance during C. elegans larval development, and three of them have apparent homologs in mammals and/or insects. Small noncoding RNAs of the miRNA class appear to be numerous and diverse.