Rapid alterations of gene expression and cytosine methylation in newly synthesized Brassica napus allopolyploids
Rapid alterations of gene expression and cytosine methylation in newly synthesized Brassica napus allopolyploids
复制标题
新合成的甘蓝型油菜异源多倍体中基因表达和胞嘧啶甲基化的快速变化
DOI：
10.1007/s00425-008-0844-8
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发表时间：
2009-02-01
期刊：
影响因子：
4.3
通讯作者：
Wang, Jianbo
中科院分区：
文献类型：
--
作者：
Xu, Yanhao;Zhong, Lan;Wang, Jianbo
异源多倍性是一种重要的物种形成机制，在植物中普遍存在。甘蓝型油菜是研究异源多倍体基因组杂交和多倍体化结果的模式系统。本研究以两组植物材料为材料，研究了异源多倍体形成早期的转录组学和表观遗传学变化。第一个比较是在合成的B之间。甘蓝型油菜异源四倍体及其二倍体祖先B. rapa（AA基因组）和B.甘蓝（CC基因组）。使用cDNA扩增片段长度多态性（cDNA-AFLP）和甲基化敏感性扩增多态性（MSAP）方法，约4.09%和6.84%的序列显示合成B的基因表达和DNA甲基化的变化。与其二倍体祖先相比。C基因组特异性基因沉默和DNA甲基化改变的比例显著大于A基因组特异性改变的比例。第二个比较是在双单倍体和双二倍体B之间。在合成的二型植物上生长的油菜器官。约0.73%的cDNA-AFLP片段和1.94%的MSAP片段显示基因表达和DNA甲基化的变化。我们对103个片段进行了测序，这些片段在合成/亲本或双单倍体/双二倍体cDNA-AFLP和MSAP比较中存在差异。序列分析显示这些片段参与多种生物学途径。我们的研究结果为人工合成B杂交和多倍化后立即发生的基因表达和DNA甲基化的全基因组变化提供了证据。油菜。此外，本研究有助于阐明基因组加倍效应对B中转录组和表观遗传学反应的影响。油菜。
Allopolyploidy is an important speciation mechanism and is ubiquitous among plants. Brassica napus is a model system for studying the consequences of hybridization and polyploidization on allopolyploid genome. In this research, two sets of plant materials were used to investigate the transcriptomic and epigenetic changes in the early stages of allopolyploid formation. The first comparison was between a synthetic B. napus allotetraploid and its diploid progenitors, B. rapa (AA genome) and B. oleracea (CC genome). Using cDNA-amplified fragment length polymorphism (cDNA-AFLP) and methylation-sensitive amplification polymorphism (MSAP) approaches, ~4.09 and 6.84% of the sequences showed changes in gene expression and DNA methylation in synthesized B. napus compared to its diploid progenitors. The proportions of C-genome-specific gene silencing and DNA methylation alterations were significantly greater than those of A-genome-specific alterations. The second comparison was between amphihaploid and amphidiploid B. napus organs grown on synthesized dimorphic plants. About 0.73% of the cDNA-AFLP fragments and 1.94% of the MSAP fragments showed changes in gene expression and DNA methylation. We sequenced 103 fragments that differed in the synthetic/parental or the amphihaploid/amphidiploid cDNA-AFLP and MSAP comparisons. Sequence analysis revealed these fragments were involved in various biological pathways. Our results provided evidence for genome-wide changes in gene expression and DNA methylation occurring immediately after hybridization and polyploidization in synthetic B. napus. Moreover, this study contributed to the elucidation of genome doubling effects on responses of transcriptome and epigenetics in B. napus.