The role of Aspergillus nidulans polo-like kinase PlkA in microtubule-organizing center control.
The role of Aspergillus nidulans polo-like kinase PlkA in microtubule-organizing center control.
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DOI：
10.1242/jcs.256537
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发表时间：
2021-07
影响因子：
4
通讯作者：
Xiaolei Gao;S. Herrero;Valentin Wernet;S. Erhardt;O. Valerius;G. Braus;R. Fischer
中科院分区：
文献类型：
--
作者：
Xiaolei Gao;S. Herrero;Valentin Wernet;S. Erhardt;O. Valerius;G. Braus;R. Fischer
中心体是动物细胞中重要的微管组织中心。此外，在许多细胞类型中描述了非中心体MTOC（ncMTOC）。真菌中中心体的功能类似物是纺锤体极体（SPB）。在构巢曲霉中，在隔膜处发现了额外的MTOC（sMTOC）。虽然核心组件在两个MTOC中都是保守的，但它们的组成和组织是不同的，而且是动态的。在这里，我们表明polo样激酶PlkA结合γ-微管蛋白环复合物（γ-TuRC）受体蛋白ApsB，并有助于将ApsB靶向两种MTOC。PlkA协调SPB外斑块与sMTOC活性。PlkA激酶活性是涉及ApsB募集的星形MT形成所必需的。PlkA还与γ-TuRC内斑受体蛋白PcpA相互作用。有丝分裂被延迟，没有PlkA，和PlkA蛋白所需的适当的有丝分裂纺锤体形态，虽然这种功能是独立的催化活性。我们的研究结果表明，polo样激酶作为MTOC活动的调节剂，并通过与γ-微管蛋白环复合物受体相互作用作为支架单元。
Centrosomes are important microtubule-organizing centers (MTOC) in animal cells. In addition, non-centrosomal MTOCs (ncMTOCs) were described in many cell types. Functional analogs of centrosomes in fungi are the spindle pole bodies (SPBs). In Aspergillus nidulans additional MTOCs were discovered at septa (sMTOC). Although the core components are conserved in both MTOCs, their composition and organization are different and dynamic. Here, we show that the polo-like kinase PlkA binds the γ-tubulin ring complex (γ-TuRC) receptor protein ApsB and contributes to targeting ApsB to both MTOCs. PlkA coordinates SPB outer plaque with sMTOC activities. PlkA kinase activity was required for astral MT formation involving ApsB recruitment. PlkA also interacted with the γ-TuRC inner plaque receptor protein PcpA. Mitosis was delayed without PlkA, and the PlkA protein was required for proper mitotic spindle morphology, although this function was independent of its catalytic activity. Our results suggest polo-like kinase as a regulator of MTOC activities and as a scaffolding unit through interaction with γ-tubulin ring complex receptors.