In vivo tissue engineering of functional skeletal muscle by freshly isolated satellite cells embedded in a photopolymerizable hydrogel
In vivo tissue engineering of functional skeletal muscle by freshly isolated satellite cells embedded in a photopolymerizable hydrogel
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DOI：
10.1096/fj.10-174755
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发表时间：
2011-07
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通讯作者：
C. Rossi;Marina Flaibani;B. Blaauw;M. Pozzobon;E. Figallo;C. Reggiani;L. Vitiello;N. Elvassore;P. de Coppi
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作者：
C. Rossi;Marina Flaibani;B. Blaauw;M. Pozzobon;E. Figallo;C. Reggiani;L. Vitiello;N. Elvassore;P. de Coppi
骨骼肌重建的成功取决于找到最有效的，临床上合适的策略，工程肌细胞和生物相容性支架。卫星细胞（SC），新鲜分离或移植到其生态位内，目前被认为是肌肉再生的最佳来源。在这里，我们设计并开发了通过原位光交联透明质酸基水凝胶、透明质酸-光引发剂（HA-PI）复合物递送SC或肌肉祖细胞（MPC）。移植有包埋在水凝胶中的新鲜分离的卫星细胞的C57 BL/6 J小鼠的部分消融的胫骨前肌（TA）与接受水凝胶+MPC或单独水凝胶的肌肉相比显示肌肉结构和新肌纤维数量的重大改善。值得注意的是，包埋在HA-PI中的SC也促进了功能恢复，如通过收缩力测量所评估的。组织重建与神经和血管网络的形成和功能SC生态位的重建有关。这种创新的方法可以克服骨骼肌组织工程以前的局限性。罗西角一、Flaibani，M.，Blaauw，B.，Pozzobon，M.，Figallo，E.，Reggiani角，维蒂耶洛湖Elvassore，N.，De Coppi，P.通过嵌入光聚合水凝胶中的新鲜分离的卫星细胞进行功能性骨骼肌的体内组织工程。FASEB J. 25，2296 - 2304（2011）。www.fasebj.org
The success of skeletal muscle reconstruction depends on finding the most effective, clinically suitable strategy to engineer myogenic cells and biocompatible scaffolds. Satellite cells (SCs), freshly isolated or transplanted within their niche, are presently considered the best source for muscle regeneration. Here, we designed and developed the delivery of either SCs or muscle progenitor cells (MPCs) via an in situ photo‐cross‐linkable hyaluronan‐based hydrogel, hyaluronic acid‐photoinitiator (HA‐PI) complex. Partially ablated tibialis anterior (TA) of C57BL/6J mice engrafted with freshly isolated satellite cells embedded in hydrogel showed a major improvement in muscle structure and number of new myofibers, compared to muscles receiving hydrogel + MPCs or hydrogel alone. Notably, SCs embedded in HA‐PI also promoted functional recovery, as assessed by contractile force measurements. Tissue reconstruction was associated with the formation of both neural and vascular networks and the reconstitution of a functional SC niche. This innovative approach could overcome previous limitations in skeletal muscle tissue engineering.—Rossi, C. A., Flaibani, M., Blaauw, B., Pozzobon, M., Figallo, E., Reggiani, C., Vitiello, L., Elvassore, N., De Coppi, P. In vivo tissue engineering of functional skeletal muscle by freshly isolated satellite cells embedded in a photopolymerizable hydrogel. FASEB J. 25, 2296‐2304 (2011). www.fasebj.org