Microbial Transglutaminase Estimation in Enzyme‐treated Surimi‐Based Products by Enzyme Immunosorbent Assay
Microbial Transglutaminase Estimation in Enzyme‐treated Surimi‐Based Products by Enzyme Immunosorbent Assay
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通过酶免疫吸附测定法估算酶处理的鱼糜产品中的微生物转谷氨酰胺酶
DOI：
10.1111/j.1365-2621.1996.tb14730.x
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发表时间：
1996
影响因子：
3.9
通讯作者：
M. Motoki
中科院分区：
文献类型：
--
作者：
T. Ohtsuka;K. Seguro;M. Motoki
利用微生物转谷氨酰胺酶(MTGase)的多克隆抗体和单抗建立了特异性检测MTGase的酶联免疫吸附试验(ELISA)。该方法以十二烷基硫酸钠变性的MTGase为标准品，检测下限为0.1μg/mL。当产品用十二烷基硫酸钠和2-巯基乙醇提取时，确保了最佳的蛋白质回收，该方法达到了测定鱼糜制品中MTGase的预期水平。在Kamaboko模型产品中，回收率为50%-71%。当该方法应用于商业产品时，在产品中发现0.01-0.18%(w/w)的MTGase。
An enzyme-linked immunosorbent assay (ELISA) for specific determination of microbial transglutaminase (MTGase) has been developed using its polyclonal and monoclonal antibodies. In the assay we calibrated sodium dodecylsulfate (SDS)-denatured MTGase for standard, and the detection limit of the SDS-denatured MTGase was 0.1 μg/mL. When products were extracted with SDS and 2-mercaptoethanol, which assured optimum protein recovery, the assay covered the expected levels for determination of MTGase in surimi-based products. In model kamaboko products, recoveries were 50-71%. When the procedures were applied to commercial products, 0.01-0.18% (w/w) of MTGase were found in the products.