The Golgi comprises a paired stack that is separated at G2 by modulation of the actin cytoskeleton through Abi and Scar/WAVE
The Golgi comprises a paired stack that is separated at G2 by modulation of the actin cytoskeleton through Abi and Scar/WAVE
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DOI：
10.1016/j.devcel.2007.03.008
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发表时间：
2007-06-01
影响因子：
11.8
通讯作者：
Rabouille, Catherine
中科院分区：
文献类型：
--
作者：
Kondylis, Vangelis;Pannerden, Hezder E. van Nispen tot;Rabouille, Catherine
在细胞周期中，高尔基体和其他细胞器一样，必须在质量和数量上进行复制，以确保其在两个子细胞之间正确分离。然而，尚不清楚何时以及如何发生这种情况。在这里，我们表明，在果蝇S2细胞中，高尔基体可能会大量复制，在G1/S期形成配对结构，并保持这种状态，直到G2时，两个堆栈分离，准备进入有丝分裂。我们表明，配对需要完整的肌动蛋白细胞骨架，而肌动蛋白细胞骨架又取决于Abi/Scar，而不是WASP。这种肌动蛋白依赖的配对不仅限于果蝇，也发生在哺乳动物细胞中。我们进一步表明，防止高尔基体堆叠分离在G2块进入有丝分裂，这表明这种配对的组织是有丝分裂检查点的一部分，类似于在哺乳动物细胞中提出的。
During the cell cycle, the Golgi, like other organeiles, has to be duplicated in mass and number to ensure its correct segregation between the two daughter cells. It remains unclear, however, when and how this occurs. Here we show that in Drosophila S2 cells, the Golgi likely duplicates in mass to form a paired structure during G1/S phase and remains so until G2 when the two stacks separate, ready for entry into mitosis. We show that pairing requires an intact actin cytoskeleton which in turn depends on Abi/Scar but not WASP. This actin-dependent pairing is not limited to flies but also occurs in mammalian cells. We further show that preventing the Golgi stack separation at G2 blocks entry into mitosis, suggesting that this paired organization is part of the mitotic checkpoint, similar to what has been proposed in mammalian cells.