Effects of temperature, plant growth regulators and substrates and changes in carbohydrate content during bulblet formation by twin scale propagation in Hippeastrum vittatum ‘Red lion’
Effects of temperature, plant growth regulators and substrates and changes in carbohydrate content during bulblet formation by twin scale propagation in Hippeastrum vittatum ‘Red lion’
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DOI：
10.1016/j.scienta.2013.06.001
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发表时间：
2013-08
影响因子：
4.3
通讯作者：
Wei Zhang-;Lina Song;J. Silva;Hongmei Sun
中科院分区：
文献类型：
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作者：
Wei Zhang-;Lina Song;J. Silva;Hongmei Sun
不同介质、温度和植物生长调节剂对朱顶红双鳞插条离体繁殖的影响以及淀粉降解与鳞茎发育的关系。研究了“红狮”。锯末是双鳞插条繁殖的最佳介质，导致腐鳞百分比最低，鳞茎质量最好。此外，25°C 是比 30 或 20°C 更合适的温度。赤霉酸（GA3）、吲哚-3-丁酸（IBA）或1-萘乙酸（NAA）不会导致鳞片繁殖和鳞茎发育。特别是，NAA 处理显着增加了鳞片腐烂的频率。在鳞茎形成和发育过程中，母鳞中淀粉含量下降较早且较快，尤其是双鳞外鳞。 30°C 下的鳞片培养导致鳞片中淀粉消耗量明显增加。鳞茎中的淀粉含量在发育过程中增加。 25°C 时鳞茎中的淀粉积累在发育的最后阶段最为显着。 H.球茎中主要可溶性糖。鳞片中含有果糖、蔗糖和葡萄糖，其中母鳞中果糖含量最高。然而，鳞茎中的关键可溶性糖是蔗糖，其含量在前 50 天内线性增加。
The effects of different media, temperatures and plant growth regulators on theex vitropropagation of twin-scale cuttings as well as on the relationship between starch degradation and bulblet development ofHippeastrum vittatumcv. ‘Red lion’ were studied. Sawdust was the most optimal medium for the propagation of twin-scale cuttings resulting in the lowest percentage of rotting scales and the best quality bulblets. Furthermore, 25 °C was a more suitable temperature than 30 or 20 °C. Gibberellic acid (GA3), indole-3-butyric acid (IBA) or 1-naphthyleneacetic acid (NAA) did not result in scale propagation and bulblet development. In particular, NAA treatment significantly increased the frequency of rotten scales. During bulblet formation and development, starch content in the mother scales declined earlier and more rapidly, especially in the outer scale of twin scales. The culture of scales at 30 °C resulted in a distinct increase in the consumption of starch in the scale. The starch content in bulblets increased during development. Starch accumulation in bulblets at 25 °C was most remarkable at the last stage of development. The dominant soluble sugars in the bulbs ofH. vittatumwere fructose, sucrose and glucose, fructose content being highest in the mother scales. However, the key soluble sugar in the bulblets was sucrose and its content increased linearly over the first 50 days.