Molecular targets of vertebrate segmentation:: Two mechanisms control segmental expression of Xenopus hairy2 during somite formation
Molecular targets of vertebrate segmentation:: Two mechanisms control segmental expression of Xenopus hairy2 during somite formation
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DOI：
10.1016/s1534-5807(01)00054-5
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发表时间：
2001-10-01
影响因子：
11.8
通讯作者：
Kirschner, MW
中科院分区：
文献类型：
--
作者：
Davis, RL;Turner, DL;Kirschner, MW
脊椎动物毛状基因的表达模式被认为是在前体中胚层的“分段时钟”的读数。在这里，我们使用转基因非洲爪蟾胚胎表明，两种类型的调控元件需要重建非洲爪蟾hairy2的节段模式。第一个是启动子元件，其含有两个针对爪蟾Su（H）的结合位点，爪蟾Su（H）是Notch靶基因的转录激活因子。第二个是hairy2 3'非翻译区（UTR）中的短序列，其最有可能在转录后起作用以调节hairy2 RNA水平。其他与毛发相关的节段表达基因的3'UTR可以替代hairy2的UTR。我们的研究结果表明，一种新的机制调节Notch靶基因的节段模式，并建议脊椎动物分割需要两个监管途径的交叉。
Vertebrate hairy genes are expressed in patterns thought to be readouts of a "segmentation clock" in the presomitic mesoderm. Here we use transgenic Xenopus embryos to show that two types of regulatory elements are required to reconstitute the segmental pattern of Xenopus hairy2. The first is a promoter element containing two binding sites for Xenopus Su(H), a transcriptional activator of Notch target genes. The second is a short sequence in the hairy2 3' untranslated region (UTR), which most likely functions post-transcriptionally to modulate hairy2 RNA levels. 3' UTRs of other hairy-related, segmentally expressed genes can substitute for that of hairy2. Our results demonstrate a novel mechanism regulating the segmental patterns of Notch target genes and suggest that vertebrate segmentation requires the intersection of two regulatory pathways.