Prevalence of Burkholderia sp nodule symbionts on four mimosoid legumes from Barro Colorado Island, Panama
Prevalence of Burkholderia sp nodule symbionts on four mimosoid legumes from Barro Colorado Island, Panama
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DOI：
10.1016/j.syapm.2004.09.002
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发表时间：
2005-01-01
影响因子：
3.4
通讯作者：
Parker, MA
中科院分区：
文献类型：
--
作者：
Barrett, CF;Parker, MA
16S rRNA和部分23S rRNA基因序列分析及基因型特异性引物PCR分析表明，在巴拿马巴罗科罗拉多岛，4种含羞草豆科植物（Mimosa pigra、M. casta、M. pudica和Abarema macradenia）的根瘤共生体中，Burkholderia属细菌占主导地位。在51个分离株中，44个是伯克氏菌，其余的是根菌、中根瘤菌或缓生根瘤菌。伯克霍尔德菌显示出4种不同的rRNA序列类型，其中23S rRNA相似度为89% ~ 97%，16S rRNA相似度为96.5 ~ 98.4%。最常见的基因型占所有分离株的53%，与三种豆科寄主物种有关。所有伯克霍尔德氏菌基因型都在阿托普卢姆（Macroptilium atropurpureum）或含羞草（Mimosa pigra）上形成结节，从结节中重新分离的菌株的rRNA基因测序证实了与接种菌株的一致性。两种伯克氏菌基因型的氮酶α亚基基因（nifD）序列分析表明，它们与南非结核伯克氏菌STM 678的部分序列最为相似。此外，用伯克霍尔德菌nodB基因特异性引物进行PCR筛选，在大多数菌株中获得了预期的扩增产物。16S rRNA和部分23S rRNA系统发育的比较表明，树的拓扑结构明显不一致。这意味着在这个伯克霍尔德菌群体中，rRNA区域的关系可能已经被横向基因转移事件改变了。(C) 2004 Elsevier GmbH版权所有。
Sequences of 16S rRNA and partial 23S rRNA genes and PCR assays with genotype-specific primers indicated that bacteria in the genus Burkholderia were the predominant root nodule symbionts for four mimosold legumes (Mimosa pigra, M. casta, M. pudica, and Abarema macradenia) on Barro Colorado Island, Panama. Among 51 isolates from these and a fifth mimosoid host (Pithecellobium hymenaeafolium), 44 were Burkholderia strains while the rest were placed in Rhizohium, Mesorhizobium, or Bradyrhizobium. The Burkholderia strains displayed four distinct rRNA sequence types, ranging from 89% to 97% similarity for 23S rRNA and 96.5-98.4% for 16S rRNA. The most common genotype comprised 53% of all isolates sampled and was associated with three legume host species. All Burkholderia genotypes formed nodules on Macroptilium atropurpureum or Mimosa pigra, and sequencing of rRNA genes in strains re-isolated from nodules verified identity with inoculant strains. Sequence analysis of the nitrogenase alpha-subunit gene (nifD) in two of the Burkholderia genotypes indicated that they were most similar to a partial sequence from the nodule-forming strain Burkholderia tuberum STM 678 from South Africa. In addition, a PCR screen with primers specific to Burkholderia nodB genes yielded the expected amplification product in most strains. Comparison of 16S rRNA and partial 23S rRNA phylogenies indicated that tree topologies were significantly incongruent. This implies that relationships across the rRNA region may have been altered by lateral gene transfer events in this Burkholderia population. (C) 2004 Elsevier GmbH. All rights reserved.