Strain variation in gene expression impact of hyphal cyclin Hgc1 in Candida albicans.
Strain variation in gene expression impact of hyphal cyclin Hgc1 in Candida albicans.
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DOI：
10.1093/g3journal/jkad151
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发表时间：
2023-08-30
影响因子：
2.6
通讯作者：
Mitchell, Aaron P.
中科院分区：
文献类型：
--
作者：
Sharma, Anupam;Mitchell, Aaron P.
菌丝的形成是真菌病原体白色念珠菌的关键毒力性状。菌丝形态发生依赖于细胞周期蛋白Hgc 1，它与细胞周期蛋白依赖性蛋白激酶Cdc 28一起作用，使驱动极化生长的效应物磷酸化。Hgc 1还通过其对2个转录因子Efg 1和Ume 6的影响参与基因调控。在这里，我们报告了对2对hgc 1 Δ/Δ突变体及其各自野生型菌株的RNA测序（RNA-seq）分析，这些突变体位于2种不同的遗传背景中。我们发现hgc 1 Δ/Δ突变改变了两种遗传背景中271个基因的表达，其中266个基因的上调或下调反应一致。在这2种背景中，一致性与efg 1 Δ/Δ突变观察到的相似，大于nrg 1 Δ/Δ突变观察到的一致性。基因表达反应包括Efg 1控制下的基因，如先前研究所预期。Hgc 1响应基因还包括麦角固醇生物合成基因和芽颈相关基因，这可能反映了Hgc 1和其他转录因子之间的相互作用以及Hgc 1对细胞长宽比的影响。
Formation of hyphae is a key virulence trait of the fungal pathogen Candida albicans. Hypha morphogenesis depends upon the cyclin Hgc1, which acts together with cyclin-dependent protein kinase Cdc28 to phosphorylate effectors that drive polarized growth. Hgc1 has also been implicated in gene regulation through its effects on 2 transcription factors, Efg1 and Ume6. Here, we report RNA-sequencing (RNA-seq) analysis of 2 pairs of hgc1Δ/Δ mutants and their respective wild-type strains, which lie in 2 different genetic backgrounds. We find that hgc1Δ/Δ mutations alter expression of 271 genes in both genetic backgrounds and 266 of those genes respond consistently with regard to up- or down-regulation. Consistency is similar to what has been observed with efg1Δ/Δ mutations and greater than observed with nrg1Δ/Δ mutations in these 2 backgrounds. The gene expression response includes genes under Efg1 control, as expected from prior studies. Hgc1-responsive genes also include ergosterol biosynthetic genes and bud neck-related genes, which may reflect interactions between Hgc1 and additional transcription factors as well as effects of Hgc1 on cellular length-to-width ratios.
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