MOLECULAR-DETECTION OF SORBITOL-FERMENTING ESCHERICHIA-COLI O157 IN PATIENTS WITH HEMOLYTIC-UREMIC SYNDROME
MOLECULAR-DETECTION OF SORBITOL-FERMENTING ESCHERICHIA-COLI O157 IN PATIENTS WITH HEMOLYTIC-UREMIC SYNDROME
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DOI：
10.1128/jcm.30.7.1807-1810.1992
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发表时间：
1992-07-01
影响因子：
9.4
通讯作者：
KARCH, H
中科院分区：
文献类型：
--
作者：
GUNZER, F;BOHM, H;KARCH, H
在104例溶血性尿毒症综合征患者中的26例和668例腹泻患者中的18例中鉴定出血清群O 157的产志贺样毒素大肠杆菌菌株。所有菌株通过菌落杂交与志贺样毒素I和志贺样毒素II基因序列互补的DNA探针进行鉴定，并通过生化试验和血清分型进行表征。44例患者中有17例感染E. coli O 157菌株，这是不寻常的，因为它们在孵育24小时内发酵山梨醇，并对β-葡萄糖醛酸酶活性呈阳性。这些产甾醇菌株的培养液对培养的Vero细胞有很强的毒性。血清学试验和B亚单位毒素基因的限制性内切酶酶切分析表明，所有17个分离株产生志贺样毒素II。虽然通过使用分子探针，我们建立了一个高频率的甾醇发酵E。大肠杆菌O 157菌株在我们检查的病人，进一步研究这些菌株在其他地方病地区的流行情况显然是必要的。
Shiga-like toxin-producing Escherichia coli strains of serogroup O157 were identified in 26 of 104 patients with hemolytic-uremic syndrome and in 18 of 668 patients with diarrhea. All strains were identified by colony hybridization with DNA probes complementary to Shiga-like toxin I and Shiga-like toxin II gene sequences and characterized by biochemical tests and serotyping. Seventeen of these 44 patients had E. coli O157 strains which were unusual because they fermented sorbitol within 24 h of incubation and were positive for beta-glucuronidase activity. Culture filtrates of these sorbitol-fermenting strains were highly toxic to Vero cells in culture. Serological tests and DNA analysis performed by restriction endonuclease digestion of B-subunit toxin genes revealed that all 17 isolates produced Shiga-like toxin II. Although by using molecular probes we established a high frequency of sorbitol-fermenting E. coli O157 strains in the patients we examined, further studies on the prevalence of such isolates in other areas of endemic disease are clearly warranted.