Autoantibodies to the heat-shock protein hsp90 in systemic lupus erythematosus.
Autoantibodies to the heat-shock protein hsp90 in systemic lupus erythematosus.
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DOI：
10.1172/jci113280
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
S. Minota;S. Koyasu;I. Yahara;J. Winfield
中科院分区：
文献类型：
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作者：
S. Minota;S. Koyasu;I. Yahara;J. Winfield
系统性红斑狼疮(SLE)患者会产生多种自身抗体。对这种自身免疫性疾病和相关自身免疫性疾病自身抗体系统的分析可以提供病因学和病理学意义的信息。我们在这里报告了一种先前未知的针对90,000-D热休克蛋白的自身抗体，HSP90，一个被认为在细胞对应激反应、病毒诱导的转化、类固醇激素受体作用和细胞激活中具有重要功能的分子。HSP90自身抗体完全属于免疫球蛋白G类，在约50%的未经选择的SLE患者和2/6的特发性多发性肌炎患者中可检测到。10例正常人、10例类风湿性关节炎、7例硬皮病患者血清中均未检测到抗HSP90抗体。这种主要的胞浆内抗原是通过用单特异性兔抗HSP90免疫吸收后从非离子洗涤剂细胞裂解物中特异性去除，以及在45摄氏度的10分钟热休克后合成增加而确定的。这些数据定义了一种针对主要热休克蛋白的新型自身抗体在SLE患者中的频繁出现。
Patients with systemic lupus erythematosus (SLE) develop multiple autoantibodies to self-antigens. Analysis of autoantibody systems in this and related autoimmune disorders can provide information of etiologic and pathogenetic significance. We report here a previously unrecognized autoantibody to the 90,000-D heat-shock protein, hsp90, a molecule thought to have important functions in the cellular response to stress, virus-induced transformation, steroid hormone receptor action, and cellular activation. Autoantibodies to hsp90 were exclusively of the IgG class, and were detected in approximately 50% of unselected patients with SLE and 2/6 patients with idiopathic polymyositis. Anti-hsp90 antibodies were not detected in sera from 10 normal subjects, 10 patients with rheumatoid arthritis, or 7 patients with scleroderma. The identity of this major intracytoplasmic antigen was established by its specific removal from nonionic detergent cell lysates following immunoabsorption with monospecific rabbit anti-hsp90, and by demonstration of increased synthesis following a 10-min 45 degrees C heat shock. These data define the frequent occurrence of a novel autoantibody to a major heat-shock protein in patients with SLE.