PINCH expression in relation to radiation response in co-cultured colon cancer cells and in rectal cancer patients
PINCH expression in relation to radiation response in co-cultured colon cancer cells and in rectal cancer patients
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DOI：
10.3892/or.2013.2673
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发表时间：
2013-11-01
期刊：
影响因子：
4.2
通讯作者：
Sun, Xiao-Feng
中科院分区：
文献类型：
--
作者：
Holmqvist, Annica;Holmlund, Birgitta;Sun, Xiao-Feng
特别有趣的是，一种新的富含半胱氨酸-组氨酸的蛋白（PINCH），参与细胞的扩散、运动和增殖，已被证明可以增强结肠癌细胞系的辐射抗性。在共培养结肠癌细胞中研究了PINCH与辐射的关系。此外，我们还分析了在有放疗或没有放疗的直肠癌患者中，PINCH与放疗（RT）的临床意义。通过western blotting和real-time PCR检测单独培养和共培养的结肠癌（KM12C）细胞中PINCH的相对表达，并在放疗后8和24 h进行分析。免疫组织化学检测了137例原发直肠肿瘤中PINCH的表达，其中65例未接受放疗，72例接受了放疗。在未接受放疗的KM12C单独培养细胞中，8 h时PINCH的表达有下降趋势（P=0.060）；而在共培养细胞中，差异无统计学意义（P=0.446）。与TNM分期、分化程度、年龄、p53状态无关，在RT患者中，与表达弱的患者相比，PINCH表达强的患者生存率较差（P=0.029, RR 4.03, 95% CI 1.34-12.1）。未接受RT治疗的患者无生存关系（P=0.287）。经统计学交互分析，PINCH、RT与生存率有显著差异（P=0.057）。综上所述，PINCH可以预测接受放疗的直肠癌患者的生存，但不能预测未接受放疗的患者的生存。PINCH的表达可能受到辐射和细胞周围环境因素的调节。
Particularly interesting new cysteine-histidine rich protein (PINCH), involved in cell spreading, motility and proliferation, has been shown to enhance radioresistance in colon cancer cell lines. The expression of PINCH in relation to radiation was studied in co-cultured colon cancer cells. Furthermore, the clinical significance between PINCH and radiotherapy (RT) was analyzed in rectal cancer patients with or without RT. The relative PINCH expression in colon cancer (KM12C) cells cultured separately and in co-culture was examined by western blotting and real-time PCR, and was analyzed over a period of 8 and 24 h after radiation. PINCH expression was immunohistochemically examined in 137 primary rectal tumors for which 65 cases did not receive RT and 72 cases received RT. PINCH expression tended to decrease from that in the separately cultured KM12C cells without radiation to that in cells with radiation at 8 h (P=0.060); while in the co-cultured cells, no significant difference was found (P=0.446). In patients with RT, strong PINCH expression was related to worse survival, when compared to patients with weak expression, independent of TNM stage, degree of differentiation, age and p53 status (P=0.029, RR 4.03, 95% CI 1.34-12.1). No survival relationship for the patients without RT was observed (P=0.287). A statistical interaction analysis between PINCH, RT and survival showed a trend towards significance (P=0.057). In conclusion, PINCH predicts survival in rectal cancer patients with RT, but not in patients without RT. The expression of PINCH may be regulated by radiation and by environmental factors surrounding the cells.