Active genes dynamically colocalize to shared sites of ongoing transcription
Active genes dynamically colocalize to shared sites of ongoing transcription
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DOI：
10.1038/ng1423
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发表时间：
2004-10-01
期刊：
影响因子：
30.8
通讯作者：
Fraser, P
中科院分区：
文献类型：
--
作者：
Osborne, CS;Chakalova, L;Fraser, P
许多基因的核内位置与其活性状态相关，这表明迁移到功能亚室可能影响基因表达。事实上，新生RNA生产和RNA聚合酶II似乎定位在离散的焦点或“转录工厂”中。目前对培养细胞的估计表明，多个基因可能占据同一个工厂，尽管尚未观察到这一点。在这里，我们表明，在体内转录过程中，远端基因以高频率共定位到同一个转录工厂。活性基因被动态地组织到共享的核亚室中，进出这些工厂导致转录的激活或减弱。因此，活性基因迁移到预组装的转录位点，而不是招募和组装转录复合物。
The intranuclear position of many genes has been correlated with their activity state, suggesting that migration to functional subcompartments may influence gene expression. Indeed, nascent RNA production and RNA polymerase II seem to be localized into discrete foci or '`transcription factories'. Current estimates from cultured cells indicate that multiple genes could occupy the same factory, although this has not yet been observed. Here we show that, during transcription in vivo, distal genes colocalize to the same transcription factory at high frequencies. Active genes are dynamically organized into shared nuclear subcompartments, and movement into or out of these factories results in activation or abatement of transcription. Thus, rather than recruiting and assembling transcription complexes, active genes migrate to preassembled transcription sites.