I-TASSER: a unified platform for automated protein structure and function prediction.
I-TASSER: a unified platform for automated protein structure and function prediction.
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DOI：
10.1038/nprot.2010.5
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发表时间：
2010-04
期刊：
影响因子：
14.8
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中科院分区：
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I-Tasser服务器是一个基于序列到结构到功能范例的自动化蛋白质结构和功能预测的集成平台。I-tasser从氨基酸序列开始，首先通过多条线对齐和迭代结构组装模拟生成三维原子模型。然后，通过将3D模型与其他已知蛋白质进行结构匹配来推断该蛋白质的功能。典型服务器运行的输出包括全长二级和三级结构预测，以及关于配体结合位点、酶委号和基因本体论术语的功能注释。基于建模的置信度分数来提供对预测的准确性的估计。该协议为设计最先进的蛋白质结构和功能预测的在线服务器系统提供了新的见解和指导方针。该服务器可在http://zhang.bioinformatics.ku.edu/I-TASSER.上获得
The I-TASSER server is an integrated platform for automated protein structure and function prediction based on the sequence-to-structure-to-function paradigm. Starting from an amino acid sequence, I-TASSER first generates three-dimensional atomic models from multiple threading alignments and iterative structural assembly simulations. The function of the protein is then inferred by structurally matching the 3D models with other known proteins. The output from a typical server run contains full-length secondary and tertiary structure predictions, and functional annotations on ligand-binding sites, Enzyme Commission numbers and Gene Ontology terms. An estimate of accuracy of the predictions is provided based on the confidence score of the modeling. This protocol provides new insights and guidelines for designing of on-line server systems for the state-of-the-art protein structure and function predictions. The server is available at http://zhang.bioinformatics.ku.edu/I-TASSER.