Relationship Between Milk Fat Globule-Epidermal Growth Factor 8 and Intestinal Cytokines in Infants Born Preterm
Relationship Between Milk Fat Globule-Epidermal Growth Factor 8 and Intestinal Cytokines in Infants Born Preterm
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DOI：
10.1016/j.jpeds.2020.11.014
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发表时间：
2021-02-22
影响因子：
5.1
通讯作者：
Codipilly, Champa N.
中科院分区：
文献类型：
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作者：
Asaro, Joseph A.;Khan, Zarak;Codipilly, Champa N.
目的探讨早产儿膳食摄入量与粪便中乳脂球-表皮生长因子8（MFG-E8）浓度之间的关系，以及粪便中MFG-E8浓度与肠道炎症标志物之间的关系。MFG-E8在肠内喂养和粪便中，粪便中的细胞因子浓度进行了定量的酶联免疫吸附试验。结果牛奶MFG-E8浓度显着更大的未强化的母亲自己的牛奶（ESTA）和ESTA与人乳强化剂比捐赠人的牛奶或早产儿配方。粪便样品中的MFG-E8浓度与相应乳汁中的MFG-E8浓度呈正相关。高MFG-E8暴露（>= 60 mL/kg/天的喂养，包括含人乳强化剂的母乳喂养或母乳喂养）与促炎细胞因子浓度较低相关（白细胞介素-8、肿瘤坏死因子-α和单核细胞趋化蛋白-1）和粪便中较高浓度的抗炎细胞因子白细胞介素-4，结论与其他饮食或不喂养的早产儿相比，喂养母乳的早产儿粪便样本中MFG-E8浓度较高，促炎细胞因子浓度较低。这些数据进一步支持可能由于MFG-E8的缘故，E10对肠道炎症的保护作用。
Objectives To investigate the relationships between dietary intake and fecal concentrations of milk fat globule-epidermal growth factor 8 (MFG-E8), and between fecal concentrations of MFG-E8 and markers of intestinal inflammation in infants born preterm.Study design Fecal samples were collected daily and enteral feedings were sampled weekly. MFG-E8 in enteral feedings and feces, and cytokine concentrations in feces were quantified by enzyme-linked immunosorbent assay.Results Milk MFG-E8 concentrations were significantly greater in unfortified mother's own milk (MOM) and MOM with human milk fortifier than either donor human milk or preterm formula. MFG-E8 concentrations in fecal samples were positively correlated with MFG-E8 concentrations in respective milks. High MFG-E8 exposure (>= 60 mL/kg/day of feedings that include MOM or MOM with human milk fortifier) was associated with lower concentrations of proinflammatory cytokines (interleukin-8, tumor necrosis factor-alpha, and monocyte chemoattractant protein-1) and higher concentrations of the anti-inflammatory cytokine interleukin-4 in feces, compared with low MFG-E8 exposure.Conclusions Infants born preterm who were fed MOM had greater concentrations of MFG-E8 and lower concentrations of proinflammatory cytokines in fecal samples than other diets or no feedings. These data further support the protective role of MOM, possibly because of MFG-E8, against intestinal inflammation.