Postmenopausal changes in production of type 1 and type 2 cytokines and the effects of hormone replacement therapy
Postmenopausal changes in production of type 1 and type 2 cytokines and the effects of hormone replacement therapy
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DOI：
10.1097/00042192-200107000-00008
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发表时间：
2001-07-01
影响因子：
2.7
通讯作者：
Aono, T
中科院分区：
文献类型：
--
作者：
Deguchi, K;Kamada, M;Aono, T
目的：对感染因子或恶性细胞的适当防御归因于T辅助细胞1型（细胞）和T辅助细胞2型（体液）免疫反应的精确平衡。我们调查的影响激素替代疗法（HRT）对绝经后的变化中产生的干扰素（IFN）-γ和白细胞介素（IL）-10，1型和2型cytokine.Design：这两种细胞因子测定ELISA的脂多糖刺激的全血细胞从72个未经治疗和44 HRT治疗的妇女的上清液。13名妇女进行了检查之前和期间HRT。结果：IFN-γ的生产在40多岁的妇女和绝经后的妇女显着高于年轻的妇女。然而，IFN-γ在绝经后期降至最低水平，而IL-10的产生随着年龄的增长而逐渐增加，并与绝经期平行。因此，在绝经后中期和晚期的妇女中，与1型细胞因子（IFN-γ）相比，2型细胞因子（IL-10）产生过多。在绝经早期和中期，HRT妇女的IFN-γ水平显著低于未经治疗的妇女，IL-10水平显著低于未经治疗的妇女在绝经中期和晚期。HRT诱导的IL-10的生产显着减少，并倾向于降低IFN-γ的水平。结论：IL-10的生产增加绝经后妇女。HRT可能通过改善1型和2型免疫反应的平衡来防止绝经后妇女免疫系统的畸变。
Objective: An appropriate defense against infective agents or malignant cells is attributed to the exquisitely balanced T helper 1 type (cellular) and T helper 2 type (humoral) immune reactions. We investigated the effect of hormone replacement therapy (HRT) on postmenopausal changes in the production of interferon (IFN)-gamma and interleukin (IL)-10, a type 1 and a type 2 cytokine, respectively.Design: Both cytokines were measured by ELISA in the supernatant of lipopolysaccharide-stimulated whole blood cells from 72 untreated and 44 HRT-treated women. Thirteen women were examined before and during HRT.Results: The production of IFN-gamma in women in their 40s and in postmenopausal women was significantly higher compared with that of younger women. However, IFN-gamma fell to the lowest level in the late postmenopausal stage, whereas the production of IL-10 increased gradually with age and in parallel with the postmenopausal period. Thus, in women in the mid- and late postmenopausal period, excessive production of type 2 cytokine (IL-10) compared with type 1 cytokine (IFN-gamma) occurred. The IFN-gamma levels of women on HRT were significantly lower than those of untreated women in the early and mid-postmenopausal stages, and IL-10 levels of women on HRT were significantly lower than those of untreated women in the mid- and late postmenopausal stages. HRT induced a significant decrease in the production of IL-10 and tended to lower the level of IFN-gamma.Conclusions: Production of IL-10 is augmented in postmenopausal women. HRT probably prevents postmenopausal women from an aberration of the immune system by improving the balance of type 1 and type 2 immune reactions.