Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells
Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells
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DOI：
10.5650/jos.ess19268
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发表时间：
2020-01-01
影响因子：
1.5
通讯作者：
Kang, Jeong-Hun
中科院分区：
文献类型：
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作者：
Asai, Daisuke;Kawano, Takahito;Kang, Jeong-Hun
溶血磷脂酰胆碱（lysoPtdCho）是由磷脂酶A（2）介导的磷脂酰胆碱水解产生的，可以刺激血管平滑肌细胞的增殖和凋亡。我们研究了培养基中胎牛血清（FBS）浓度对lysoPtdCho介导的人主动脉平滑肌细胞（HASMCs）凋亡和增殖以及细胞外信号调节激酶（ERK）1/2激活的影响。在存在1%FBS的情况下，HASMC的活力在lysoPtdCho处理后在1和10 μ M时增加，但在25和50 μ M时降低。然而，在10%FBS存在下，lysoPtdCho以剂量依赖性方式增加HASMC活力。在1%FBS存在下，25 μ M lysoPtdCho可增加HASMCs中半胱天冬酶3/7的活性，但不增加10%FBS的活性。此外，lysoPtdCho在1和10 μ M触发ERK 1/2磷酸化的存在下，1%FBS，但不是在10%FBS。因此，lysoPtdCho介导的HASMC凋亡、增殖和ERK 1/2活化依赖于FBS的浓度。
Lysophosphatidylcholine (lysoPtdCho) is produced by the phospholipase A(2)-mediated hydrolysis of phosphatidylcholine and can stimulate proliferation and apoptosis of vascular smooth muscle cells. We examined the influence of fetal bovine serum (FBS) concentration in the culture medium on lysoPtdCho-mediated apoptosis and proliferation of human aortic smooth muscle cells (HASMCs) as well as on the activation of extracellular signal-regulated kinases (ERK)1/2. In the presence of 1% FBS, HASMC viability increased after lysoPtdCho treatment at I and 10 mu M but decreased at 25 and 50 mu M. However, lysoPtdCho increased HASMC viability in a dose-dependent manner in the presence of 10% FBS. The activity of caspase 3/7 in HASMCs was increased by 25 mu M lysoPtdCho in the presence of 1% FBS, but not 10% FBS. Furthermore, lysoPtdCho at 1 and 10 mu M triggered ERK1/2 phosphorylation in the presence of 1% FBS, but not at 10% FBS. Thus, lysoPtdCho-mediated HASMC apoptosis, proliferation, and ERK1/2 activation are dependent on the concentration of FBS.