Preclinical Validation of a Single-Scan Rest/Stress Imaging Technique for 13N-Ammonia Positron Emission Tomography Cardiac Perfusion Studies.
Preclinical Validation of a Single-Scan Rest/Stress Imaging Technique for 13N-Ammonia Positron Emission Tomography Cardiac Perfusion Studies.
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用于 13N-氨正电子发射断层扫描心脏灌注研究的单扫描静息/应激成像技术的临床前验证。
DOI：
10.1161/circimaging.119.009407
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发表时间：
2020
期刊：
影响因子：
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通讯作者：
Alpert,NathanielM
中科院分区：
文献类型：
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作者：
Guehl,NicolasJ;Pelletier-Galarneau,Matthieu;Wooten,DustinW;Guerrero,JLuis;Kas,Aurélie;Normandin,MarcD;Fakhri,GeorgesEl;Alpert,NathanielM
背景我们以前提出了一种技术，用于定量测量静息和负荷绝对心肌血流量（MBF）使用2注射单扫描成像会话。最近，我们在猪模型中验证了使用腺苷作为药理应激物的长寿命放射性示踪剂18F-Flurpiridaz的方法。本工作的目的是验证我们的技术为13 NH3。方法在6只猪进行了9项研究，其中5项研究在自然状态下进行，4项在左前降支梗死后进行。每项研究包括3次动态扫描：2次注射静息-静息单次扫描采集（扫描A）、2次注射静息/负荷单次扫描采集（扫描B）和常规1次注射负荷采集（扫描C）。给予不同剂量的腺苷联合多巴酚丁胺以诱导广泛的MBF。用我们的非平稳动力学模型（MGH 2）拟合2次注射单扫描测量。在4项研究中，13 NH3注射与微球注射配对。用我们的方法获得的MBF估计值与标准方法和微球获得的MBF估计值进行比较。结果在无应力的情况下（扫描A），MGH 2估算的2种放射性示踪剂注射的MBF值几乎相同（平均差：0.067±0.070 mL·min-1·cc-1，一致性限：[-0.070 to 0.204] mL·min-1·cc-1），显示出良好的重复性。Bland-Altman分析表明，对于两种剩余时间，（平均差异：−0.034±0.035 mL·min−1·cc−1，一致性限值：[−0.103至0.035] mL·min−1·cc−1）和应力（平均差：0.057±0.361 mL·min−1·cc−1，一致性限值：[−0.651至0.765] mL·min−1·cc−1）MBF测量。正电子发射断层扫描和微球MBF测量密切相关。非常好的质量灌注images.ConclusionsThis研究提供了在体内验证我们的单扫描静息应力方法为13 NH 3测量。13 NH3静息/负荷心肌灌注成像过程可以压缩成一个单一的正电子发射断层扫描持续时间不到15分钟。
BackgroundWe previously proposed a technique for quantitative measurement of rest and stress absolute myocardial blood flow (MBF) using a 2-injection single-scan imaging session. Recently, we validated the method in a pig model for the long-lived radiotracer18F-Flurpiridaz with adenosine as a pharmacological stressor. The aim of the present work is to validate our technique for13NH3.MethodsNine studies were performed in 6 pigs; 5 studies were done in the native state and 4 after infarction of the left anterior descending artery. Each study consisted of 3 dynamic scans: a 2-injection rest-rest single-scan acquisition (scan A), a 2-injection rest/stress single-scan acquisition (scan B), and a conventional 1-injection stress acquisition (scan C). Variable doses of adenosine combined with dobutamine were administered to induce a wide range of MBF. The 2-injection single-scan measurements were fitted with our nonstationary kinetic model (MGH2). In 4 studies,13NH3injections were paired with microsphere injections. MBF estimates obtained with our method were compared with those obtained with the standard method and with microspheres. We used a model-based method to generate separate rest and stress perfusion images.ResultsIn the absence of stress (scan A), the MBF values estimated by MGH2 were nearly the same for the 2-radiotracer injections (mean difference: 0.067±0.070 mL·min−1·cc−1, limits of agreement: [−0.070 to 0.204] mL·min−1·cc−1), showing good repeatability. Bland-Altman analyses demonstrated very good agreement with the conventional method for both rest (mean difference: −0.034±0.035 mL·min−1·cc−1, limits of agreement: [−0.103 to 0.035] mL·min−1·cc−1) and stress (mean difference: 0.057±0.361 mL·min−1·cc−1, limits of agreement: [−0.651 to 0.765] mL·min−1·cc−1) MBF measurements. Positron emission tomography and microsphere MBF measurements correlated closely. Very good quality perfusion images were obtained.ConclusionsThis study provides in vivo validation of our single-scan rest-stress method for13NH3measurements. The13NH3rest/stress myocardial perfusion imaging procedure can be compressed into a single positron emission tomography scan session lasting less than 15 minutes.