THE HIGHER-ORDER STRUCTURE OF CHICKEN ERYTHROCYTE CHROMOSOMES INVIVO
THE HIGHER-ORDER STRUCTURE OF CHICKEN ERYTHROCYTE CHROMOSOMES INVIVO
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DOI：
10.1038/288620a0
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发表时间：
1980-01-01
期刊：
影响因子：
64.8
通讯作者：
SCHUTT, C
中科院分区：
文献类型：
--
作者：
LANGMORE, JP;SCHUTT, C
小角X射线散射被用来研究活体中鸡红细胞染色体的组织，并解释了以前观察到的许多X射线和EM观察中的不一致之处。鸡红细胞染色体具有400°的周期性，这是由于其核结构直接与包埋细胞的300°并排堆积的染色体纤维有关。如果使用适当的缓冲液，这种周期性可以在孤立的原子核中保持。
Low-angle X-ray scattering was used to investigate the organization of chicken erythrocyte chromosomes in vivo and to account for the previously observed inconsistencies in many X-ray and EM observations. Chicken erythrocyte chromosomes have a 400 .ANG. periodicity due to a nuclear structure that is directly related to the 300 .ANG. side-by-side packing of chromosome fibers revealed by EM of embedded cells. This periodicity can be preserved in isolated nuclei provided that the proper buffers are used.