Acquired xanthine dehydrogenase expression shortens survival in patients with resected adenocarcinoma of lung
Acquired xanthine dehydrogenase expression shortens survival in patients with resected adenocarcinoma of lung
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DOI：
10.1007/s13277-012-0431-2
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发表时间：
2012-10-01
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影响因子：
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通讯作者：
Ogawa, Jun-ichi
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文献类型：
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作者：
Konno, Hayato;Minamiya, Yoshihiro;Ogawa, Jun-ichi
黄嘌呤脱氢酶（XDH），也称为黄嘌呤氧化还原酶（XOR），长期以来被认为是嘌呤分解代谢的关键酶，将次黄嘌呤氧化成黄嘌呤，然后将黄嘌呤氧化成尿酸。此外，据报道XDH表达水平与恶性肿瘤患者的预后相关，但肺癌临床病理特征与XDH之间的关系尚不完全清楚。因此，我们使用半定量实时逆转录聚合酶链反应来评估 88 名肺腺癌患者的肿瘤样本中 XDH mRNA 的表达。然后我们将 XDH mRNA 水平与已知的临床病理因素相关联。我们发现，强表达 XDH 的患者的 5 年总体生存率显着低于表达较低水平 XDH 的患者（P < 0.001；对数秩检验）。正常肺组织不表达 XDH。多变量 Cox 比例风险分析显示，男性（风险比，3.14；95% 置信区间 (CI)，1.45-7.07；P = 0.004）、淋巴结转移阳性（风险比，5.74；95% CI，1.94-19.3；P = 0.001）和高 XDH 表达（风险比， 2.33；95% CI，1.11-5.02；P = 0.026）都是影响 5 年无病生存的独立因素。总之，肿瘤 XDH 高表达是肺腺癌患者预后不良的独立预测因子。
Xanthine dehydrogenase (XDH), also known as xanthine oxidoreductase (XOR), has long been recognized as the key enzyme in the catabolism of purines, oxidizing hypoxanthine into xanthine and then xanthine into uric acid. In addition, levels of XDH expression are reportedly related to the prognosis of patients with malignant tumors, though the relationship between the clinicopathological features of lung cancer and XDH is not fully understood. We therefore used semiquantitative real-time reverse transcription polymerase chain reaction to assess expression of XDH mRNA in tumor samples from 88 patients with adenocarcinoma of the lung. We then correlated XDH mRNA levels with known clinicopathological factors. We found that the 5-year overall survival rate among patients strongly expressing XDH was significantly poorer than among those expressing lower levels of XDH (P < 0.001; log-rank test). Normal lung tissue does not express XDH. Multivariate Cox proportional hazard analyses revealed that being male (hazard ratio, 3.14; 95 % confidence interval (CI), 1.45-7.07; P = 0.004), nodal metastasis positivity (hazard ratio, 5.74; 95 % CI, 1.94-19.3; P = 0.001), and high XDH expression (hazard ratio, 2.33; 95 % CI, 1.11-5.02; P = 0.026) were all independent factors affecting 5-year disease-free survival. In conclusion, high tumoral XDH expression is an independent predictor of a poor prognosis in patients with adenocarcinoma of the lung.