Oxygen sensitivity and biocompatibility of an implantable paramagnetic probe for repeated measurements of tissue oxygenation
Oxygen sensitivity and biocompatibility of an implantable paramagnetic probe for repeated measurements of tissue oxygenation
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DOI：
10.1007/s10544-009-9298-4
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发表时间：
2009-08-01
影响因子：
2.8
通讯作者：
Kuppusamy, Periannan
中科院分区：
文献类型：
--
作者：
Meenakshisundaram, Guruguhan;Eteshola, Edward;Kuppusamy, Periannan
使用氧传感水不溶性顺磁探针，如八正丁氧基萘二甲酸锂（LiNc-BuO），可以通过电子顺磁共振（EPR）光谱法重复测量组织中相同位置的pO（2）。为了促进直接在体内应用，因此最终的临床应用，LiNc-BuO，我们封装LiNc-BuO微晶聚二甲基硅氧烷（PDMS），氧气渗透性和生物惰性聚合物，并开发了一种可植入的芯片。在灭菌、高能照射和暴露于培养细胞的条件下对芯片进行的体外评估表明，它具有生物稳定性和生物相容性。将芯片植入小鼠腓肠肌组织表明，它能够在较长时间内重复和实时测量组织氧合。使用小鼠肿瘤模型的功能评估建立了用于监测肿瘤氧合的芯片的适用性和适用性。本研究确立了PDMS封装的LiNc-BuO作为临床EPR血氧测定探针的有前途的选择。
The use of oxygen-sensing water-insoluble paramagnetic probes, such as lithium octa-n-butoxynaphthalocyanine (LiNc-BuO), enables repeated measurements of pO(2) from the same location in tissue by electron paramagnetic resonance (EPR) spectroscopy. In order to facilitate direct in vivo application, and hence eventual clinical applicability, of LiNc-BuO, we encapsulated LiNc-BuO microcrystals in polydimethylsiloxane (PDMS), an oxygen-permeable and bioinert polymer, and developed an implantable chip. In vitro evaluation of the chip, performed under conditions of sterilization, high-energy irradiation, and exposure to cultured cells, revealed that it is biostable and biocompatible. Implantation of the chip in the gastrocnemius muscle tissue of mice showed that it is capable of repeated and real-time measurements of tissue oxygenation for an extended period. Functional evaluation using a murine tumor model established the suitability and applicability of the chip for monitoring tumor oxygenation. This study establishes PDMS-encapsulated LiNc-BuO as a promising choice of probe for clinical EPR oximetry.