Effects of whey protein hydrolysate on growth promotion and immunomodulation in mouse pups in artificial rearing system
Effects of whey protein hydrolysate on growth promotion and immunomodulation in mouse pups in artificial rearing system
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乳清蛋白水解物对人工饲养系统幼鼠的生长促进和免疫调节作用
DOI：
10.1111/asj.13395
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发表时间：
2020
影响因子：
2
通讯作者：
Moriguchi Toru
中科院分区：
文献类型：
--
作者：
Takeda Shiro;Harauma Akiko;Okamoto Mariko;Enomoto Hirofumi;Kudo Toshiji;Suzuki Takehito;Mizunoya Wataru;Moriguchi Toru
研究乳清蛋白水解物（WPH）对人工饲养（AR）小鼠仔鼠生长发育和免疫功能的影响。小鼠幼仔在AR系统中饲养，其中人工乳汁包括5% WPH（AR + WPH）或不含WPH（AR + WPH），其余幼仔在出生后由其母亲（母鼠）饲养14天。AR伴WPH组的体重变化和体重增加率显著高于AR不伴WPH组，与母兽组相似。添加WPH组的饲料效率和蛋白质效率显著高于不添加WPH组。此外，在AR系统中添加WPH可显著提高胸腺和/或脾细胞中CD 3 + CD 8+、B 2 2 0 + CD 19+、IA/IE+ CD 11 c+和CD 11 b+的数量及胸腺重量。此外，MALDI-TOF/MS分析鉴定了对应于一些肽的氨基酸序列，并表明VRTPEVDDE在来自测试的WPH的肽中具有最高的相对强度。因此，在AR系统中，WPH不仅需要促进小鼠幼仔的生长，而且还需要发挥免疫调节活性。
This study aimed to investigate the effects of whey protein hydrolysate (WPH) on the growth and immunity of mouse pups in artificial rearing (AR) system. Mouse pups were reared in the AR system with artificial milk including 5% WPH (AR with WPH) or not (AR without WPH), and the remaining pups were reared by their mother (dam) for 14 days after birth. The body weight change and body weight gain rates in the AR with WPH group were significantly higher than those observed in the AR without WPH group and similar to those in the dam group. Moreover the feed and protein efficiencies in the AR with WPH group were significantly higher than those of the AR without WPH group. In addition, the supplement of WPH in the AR system was shown to significantly elevate the number of CD3+CD8+, B220+CD19+, IA/IE+CD11c+, and CD11b+in the thymocyte and/or splenocyte, and the thymus weight. Furthermore, MALDI‐TOF/MS analysis identified the amino acid sequences corresponding to some peptides, and indicated that VRTPEVDDE had the highest relative intensity among the peptides from tested WPH. Therefore, WPH would be required to not only promote growth, but also exert immunomodulatory activities in mouse pups in AR system.