Protein diversity in discrete structures at the distal tip of the trypanosome flagellum
Protein diversity in discrete structures at the distal tip of the trypanosome flagellum
复制标题
锥虫鞭毛远端离散结构的蛋白质多样性
DOI：
10.1073/pnas.1703553114
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
K. Gull
中科院分区：
文献类型：
--
作者：
Vladimír Varga;F. Moreira;N. Portman;K. Gull
意义 真核生物鞭毛/纤毛的远端具有关键功能，但由于其尺寸小以及尖端结构与轴丝的关联，研究起来相当困难。我们开发了生化方法来识别一组针对鞭毛尖端结构的特异性蛋白质。我们将这些蛋白质亚定位到单独的结构中。通过功能研究，我们阐明了所鉴定的蛋白质如何促进鞭毛连接器（锥虫鞭毛尖端的移动膜连接处）的功能。真核鞭毛/纤毛的远端对于轴丝生长和信号传导很重要，并且具有独特的生物力学特性。特定的鞭毛尖端结构是存在的，但它们的组成、动力学和功能在很大程度上是未知的。我们使用生化方法来识别组装锥虫鞭毛尖端的鞭毛连接器的七个成分以及组装和成熟鞭毛尖端的轴丝帽结构的三个成分。两种尖端结构都含有进化上保守的蛋白质以及动质体特异性蛋白质，并且在鞭毛延伸过程中很早就将组件组装到结构中。定位和功能研究表明，鞭毛连接器膜连接处通过驱动蛋白-15 家族成员附着在组装鞭毛的延伸微管的尖端上。在另一侧，动质体特异性驱动蛋白促进连接点与成熟鞭毛中的微管的附着。功能研究还表明了其他几个组件的作用以及尖端结构中子域的定义。
Significance The distal end of the eukaryotic flagellum/cilium has critical functions, yet due to its small dimensions and association of tip structures with the axoneme is rather intractable to studying. We have developed biochemical approaches to identify a cohort of proteins specific for the flagellum tip structures. We sublocalized these proteins into individual structures. Using functional studies, we elucidated how the identified proteins contribute to the function of the flagella connector, the mobile membrane junction at the tip of the trypanosome flagellum. The distal end of the eukaryotic flagellum/cilium is important for axonemal growth and signaling and has distinct biomechanical properties. Specific flagellum tip structures exist, yet their composition, dynamics, and functions are largely unknown. We used biochemical approaches to identify seven constituents of the flagella connector at the tip of an assembling trypanosome flagellum and three constituents of the axonemal capping structure at the tips of both assembling and mature flagella. Both tip structures contain evolutionarily conserved as well as kinetoplastid-specific proteins, and component assembly into the structures occurs very early during flagellum extension. Localization and functional studies reveal that the flagella connector membrane junction is attached to the tips of extending microtubules of the assembling flagellum by a kinesin-15 family member. On the opposite side, a kinetoplastid-specific kinesin facilitates attachment of the junction to the microtubules in the mature flagellum. Functional studies also suggest roles of several other components and the definition of subdomains in the tip structures.
影响因子：
4.5
作者：
Haycraft CJ;Banizs B;Aydin-Son Y;Zhang Q;Michaud EJ;Yoder BK
通讯作者：
Yoder BK