Cellular migration and morphology in corneal endothelial wound repair.
Cellular migration and morphology in corneal endothelial wound repair.
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发表时间：
1985-04
影响因子：
4.4
通讯作者：
Mamoru Marsuda;Mirsuru Sawa;H. Edelhauser;Stephen P. Barrels;Arthur H. Neufeld;Kenneth R. Kenyonj
中科院分区：
文献类型：
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作者：
Mamoru Marsuda;Mirsuru Sawa;H. Edelhauser;Stephen P. Barrels;Arthur H. Neufeld;Kenneth R. Kenyonj
机械剥脱兔角膜内皮细胞后，愈合过程中，随后与宽场镜面显微镜。单个细胞迁移和形态学变化进行了分析，通过计算机辅助形态计量学。伤口周围的细胞迁移到覆盖缺损处，而不产生细胞间间隙。最大的细胞迁移和形态学改变发生在伤口边缘附近。随着细胞向伤口迁移，它们在迁移方向上伸长并增加其表面积。随着愈合的进行，细胞失去了原来的六边形图案，在覆盖完成后又恢复了。伤口完全覆盖的大，不规则形状的细胞显示有丝分裂的数字之间的24和48小时。在此期间，细胞迁移减少，正常的细胞形态开始恢复。当有丝分裂减少时，正常的细胞图案重新排列成更六边形的形状。在愈合过程中，细胞迁移的程度和方向因细胞而异。此外，三分之一的迁移细胞发生了细胞间接触的变化（相邻细胞的位置变化）。这种细胞迁移可以解释单层细胞滑动而不会在单个细胞之间产生间隙。
After a mechanical denudation of rabbit corneal endothelial cells, the healing process was followed with wide-field specular microscopy. Individual cell migration and morphologic changes were analyzed by computer-assisted morphometry. The cells surrounding the wound migrated to cover the defect without producing intercellular gaps. The greatest cellular migration and morphologic alterations occurred close to the wound edge. As the cells migrated toward the wound, they elongated and increased their surface area in the direction of the migration. As the healing proceeded, the cells lost their original hexagonal pattern, which returned after coverage was complete. The wound was covered completely by large, irregularly shaped cells showing mitotic figures between 24 and 48 hr. During this period, cellular migration decreased and normal cellular morphology began to recover. When mitosis decreased, the normal cellular pattern rearranged towards a more hexagonal shape. During the healing process, the degree and direction of cellular migration varied from cell to cell. Additionally, changes in cell-to-cell contact (positional changes of neighboring cells) occurred in one-third of migrating cells. Such cellular migration can account for monolayered cells sliding without producing gaps between individual cells.