BENEFICIAL EFFECTS OF EXOGENOUS CROSSLINKING AGENTS ON SELF-ASSEMBLED TISSUE ENGINEERED CARTILAGE CONSTRUCT BIOMECHANICAL PROPERTIES
BENEFICIAL EFFECTS OF EXOGENOUS CROSSLINKING AGENTS ON SELF-ASSEMBLED TISSUE ENGINEERED CARTILAGE CONSTRUCT BIOMECHANICAL PROPERTIES
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DOI：
10.1142/s0219519410003769
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发表时间：
2011-04-01
影响因子：
0.8
通讯作者：
Athanasiou, Kyriacos A.
中科院分区：
文献类型：
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作者：
Elder, Benjamin D.;Mohan, Arvind;Athanasiou, Kyriacos A.
背景。由于关节软骨无法自我修复，临床对组织工程替代组织的需求很大。目前使用自组装过程的组织工程研究已经显示出有希望的结果，但生物力学性能仍保持在大约50%的天然组织。方法/主要结果。本研究的目的是确定使用外源性交联剂增强无支架软骨组织工程方法的生物力学性能的可行性。四种交联剂（戊二醛、核糖、吉尼平和甲基乙二醛）分别以单一浓度和单一应用时间施用。结果表明，加入核糖后，杨氏模量显著增加69%，抗拉强度显著增加47%，骨料模量也有显著增加的趋势。此外，甲基乙二醛的应用导致杨氏模量显著增加58%。没有任何处理改变组织的生化成分。结论/意义。据我们所知，这是第一次研究外源性交联剂在使用无支架组织工程方法形成的任何组织上的使用。特别是，本研究表明，一次性使用交联剂可以有效地增强组织工程关节软骨的生物力学性能。结果令人兴奋，因为它们证明了使用外源性交联剂增强生物力学性能而不需要增加糖胺聚糖（GAG）和胶原蛋白含量的可行性。
Background. As articular cartilage is unable to repair itself, there is a tremendous clinical need for a tissue engineered replacement tissue. Current tissue engineering efforts using the self-assembly process have demonstrated promising results, but the biomechanical properties remain at roughly 50% of native tissue. Methodology/Principal Findings. The objective of this study was to determine the feasibility of using exogenous crosslinking agents to enhance the biomechanical properties of a scaffoldless cartilage tissue engineering approach. Four crosslinking agents (glutaraldehyde, ribose, genipin, and methylglyoxal) were applied each at a single concentration and single application time. It was determined that ribose application resulted in a significant 69% increase in Young's modulus, a significant 47% increase in ultimate tensile strength, as well as a trend toward a significant increase in aggregate modulus. Additionally, methylglyoxal application resulted in a significant 58% increase in Young's modulus. No treatments altered the biochemical content of the tissue. Conclusions/Significance. To our knowledge, this is the first study to examine the use of exogenous crosslinking agents on any tissue formed using a scaffoldless tissue engineering approach. In particular, this study demonstrates that a one-time treatment with crosslinking agents can be employed effectively to enhance the biomechanical properties of tissue engineered articular cartilage. The results are exciting, as they demonstrate the feasibility of using exogenous crosslinking agents to enhance the biomechanical properties without the need for increased glycosaminoglycan (GAG) and collagen content.