Secretory granules of endocrine and chief cells of human stomach mucosa contain leptin
Secretory granules of endocrine and chief cells of human stomach mucosa contain leptin
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DOI：
10.1038/sj.ijo.0801228
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发表时间：
2000-06-01
影响因子：
4.9
通讯作者：
Palou, A
中科院分区：
文献类型：
--
作者：
Cinti, S;De Matteis, R;Palou, A
背景技术背景：瘦素在控制食物摄入和体重平衡中起重要作用。在人类中，瘦素由脂肪细胞、胎盘细胞和乳腺上皮的分泌细胞产生。目的：检测瘦素蛋白在人胃中的表达，并通过免疫细胞化学方法定位产生瘦素的特异性细胞类型。设计：采用逆转录聚合酶链反应（RT-PCR）技术，对6例患者胃镜活检标本进行研究。结果：人胃粘膜上皮细胞表达瘦素mRNA和瘦素蛋白。下半部分胃腺细胞呈瘦素免疫反应阳性。超微结构免疫细胞化学显示主细胞胃蛋白酶原颗粒中的瘦素免疫反应阳性，但腺体基底部的特定内分泌细胞类型的颗粒也呈阳性。我们的研究结果表明，胃瘦素可以在短-胃内分泌系统控制摄食行为，可能由主细胞在胃腔分泌，并由一种特殊类型的内分泌系统进入胃循环cell.
BACKGROUND: Leptin plays an important role in the control of food intake and body weight homeostasis, In humans, leptin is produced by adipocytes, placental cells and secretory cells of the mammary epithelium. Recently, it has been reported that stomach glands produce leptin in rats.OBJECTIVE: To test the expression of leptin protein in human stomach and localize, by immunocytochemistry, the specific cell type producing leptin.DESIGN: Endoscopic stomach biopsies of six patients were used to investigate leptin production in the fundic epithelium using reverse transcription polymerase chain reaction (RT-PCR) of RNA. Leptin protein was detected by immunoblot analysis and localized by immunohistochemistry and ultrastructural immunocytochemistry (immunogold method).RESULTS: Human gastric epithelium expresses leptin mRNA and leptin protein. The cells in the lower half of the stomach glands were immunoreactive for leptin. Ultrastructural immunocytochemistry showed leptin immunoreactivity in the pepsinogen granules of chief cells, but the granules of a specific endocrine cell type in the basal portion of the glands were also positive.CONCLUSIONS: Our results suggest that gastric leptin could function in the short-term system to control feeding behaviour and is probably secreted in the stomach lumen by chief cells and into the stomach circulation by a special type of endocrine cell.