The salicylic acid dependent and independent effects of NMD in plants.
The salicylic acid dependent and independent effects of NMD in plants.
复制标题
DOI：
10.4161/psb.21960
复制
发表时间：
2012-11
影响因子：
2.9
通讯作者：
Davies B
中科院分区：
文献类型：
--
作者：
Rayson S;Ashworth M;de Torres Zabala M;Grant M;Davies B
在真核生物中，无义介导的mRNA衰变（NMD）针对异常和选择的非异常mRNA进行破坏。最近对拟南芥nmd缺陷突变体中显示丰度增加的mrna的筛选显示，大多数mrna与水杨酸（SA）介导的防御途径有关。具有保守肽上游开放阅读框（cpuorf或cuorf）的mrna在与SA无关的较小类别的nmd调控转录物中大量存在。在这里，我们发现在拟南芥NMD突变体中观察到的常见表型依赖于SA，而NMD突变体中含有cpuorf的转录本的上调不依赖于SA。我们推测cpuorf可以通过NMD途径有条件地靶向mrna进行破坏。
In eukaryotes, nonsense-mediated mRNA decay (NMD) targets aberrant and selected non-aberrant mRNAs for destruction. A recent screen for mRNAs showing increased abundance in Arabidopsis NMD-deficient mutants revealed that most are associated with the salicylic acid (SA)-mediated defense pathway. mRNAs with conserved peptide upstream open reading frames (CpuORFs or CuORFs) are hugely overrepresented among the smaller class of NMD-regulated transcripts not associated with SA. Here we show that the common phenotypes observed in Arabidopsis NMD mutants are SA-dependent, whereas the upregulation of CpuORF-containing transcripts in NMD mutants is independent of SA. We speculate that CpuORFs could allow the conditional targeting of mRNAs for destruction using the NMD pathway.