Evidence that platelets promote tube formation by endothelial cells on matrigel
Evidence that platelets promote tube formation by endothelial cells on matrigel
复制标题
DOI：
10.1038/sj.bjp.0702191
复制
发表时间：
1998-11-01
影响因子：
7.3
通讯作者：
Maragoudakis, ME
中科院分区：
文献类型：
--
作者：
Pipili-Synetos, E;Papadimitriou, E;Maragoudakis, ME
1在体外血管生成模型基质管形成实验中，研究了血小板在新生血管形成中的作用血小板促进毛细血管样结构（以相对管面积表示）的形成与数量和时间有关。相对管面积从存在6.25 × 10(4)时的0.98+/-0.02 （n = 8）增加到存在10(6)个血小板/孔时的3.21 +/- 0.12 (n = 8)，而不存在血小板/孔时的0.54+/-0.04 （n = 8）。这种增加不受乙酰水杨酸（ASA）、嘧酶和水蛭素的影响。代表性实验的照片显示，血小板沿着分化的内皮粘附与单独使用血小板相比，加入α -凝血酶（0.1-1 iu ml(-1)）、一氧化氮（NO）供体硝普钠（SNP, 1-100 μ M）或NO合成酶抑制剂l - ng -精氨酸-甲基lester （L-NAME, 30-300 μ M）对试管的形成没有影响神经氨酸酶（0.01 iu /10(7)个血小板）从膜糖蛋白中剥离唾液酸残基，消除了血小板对输卵管形成的促进作用。神经氨酸酶处理血小板组的相对管面积为0.81+/-0.03 (n = 8)，未处理血小板组的相对管面积为1.69+/-0.09 （P = 8）
1 The involvement of platelets in neovascularization was investigated in the matrigel tube formation assay, an in vitro model of angiogenesis.2 Platelets promoted the formation of capillary-like structures (expressed as relative tube area) number- and time-dependently. Relative tube area increased from 0.98+/-0.02 (n = 8) in the presence of 6.25 x 10(4), to 3.21 +/- 0.12 (n = 8) in the presence of 10(6) platelets/well compared to 0.54+/-0.04 (n = 8) in their absence. This increase was unaffected by acetyl salicylic acid (ASA), apyrase, and hirudin. Photographs from representative experiments, showed that platelets adhered along the differentiating endothelium.3 Addition of alpha-thrombin (0.1-1 i.u. ml(-1)), the nitric oxide (NO) donor sodium nitroprusside (SNP; 1-100 mu M) or the NO synthase inhibitor, L-NG-arginine-methylester (L-NAME, 30-300 mu M) to the assay, had no effect on tube formation compared to that seen with platelets alone.4 Neuraminidase (0.01 i.u./10(7) platelets), which strips sialic acid residues from membrane glycoproteins, abolished the promoting effect of platelets on tube formation. The relative tube area in the presence of neuraminidase-treated platelets was 0.81+/-0.03 (n = 8), in the presence of untreated platelets 1.69+/-0.09, P