Neonatal manipulation of oxytocin affects expression of estrogen receptor alpha
Neonatal manipulation of oxytocin affects expression of estrogen receptor alpha
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DOI：
10.1016/j.neuroscience.2005.08.065
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发表时间：
2006-12
期刊：
影响因子：
3.3
通讯作者：
Yukiyo Yamamoto;C. Carter;B. Cushing
中科院分区：
文献类型：
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作者：
Yukiyo Yamamoto;C. Carter;B. Cushing
在成年女性中，神经肽催产素的许多作用是类固醇，特别是雌激素依赖性的。在这里，我们首次证明，新生儿催产素的操作可以影响雌激素受体α的表达。产后第一天，雄性和雌性草原田鼠（Microtus ochrogaster）被随机分配接受四种治疗之一;（a）50μl腹腔注射3μg催产素（约1μg/g），（b）0.3μg催产素拮抗剂（约0.1μg/g），或（B）等渗盐水。第四组被处理，但不注射。在出生后第8或21天，收集脑组织，固定并切片。使用免疫细胞化学对自由漂浮切片进行雌激素受体α染色，并按年龄、治疗和性别对雌激素受体α免疫反应神经元进行比较。为了比较雌激素受体α的时间表达，在出生当天从未处理的雄性和雌性收集另外一组脑。催产素操作的影响是年龄依赖性的，性二态性，和特定的网站。虽然在出生后第8天没有显著的治疗效果，但到出生后第21天，接受催产素的雌性动物显示下丘脑腹内侧核中表达雌激素受体α免疫反应性的细胞数量显著增加。催产素拮抗剂治疗导致雌激素受体α-免疫反应性显着减少，在内侧视前区在出生后第21天的女性。虽然在雄性中没有显著影响，但催产素拮抗剂处理的雄性有内侧杏仁核雌激素受体α免疫反应性降低的趋势。结果表明，催产素可以有组织的影响，雌激素受体α的表达，这些影响是性的二态性，最后，在断奶前期间的雌激素受体α的发展是性的二态性。
In adult females many of the effects of the neuropeptide oxytocin are steroid, and especially estrogen dependent. Here we demonstrate for the first time that neonatal manipulation of oxytocin can affect the expression of estrogen receptor alpha. On the first day of postnatal life male and female prairie voles (Microtus ochrogaster) were randomly assigned to receive one of four treatments; (a) 50μl i.p. injection of 3μg oxytocin (approximately 1μg/g), (b) 0.3μg of an oxytocin antagonist (approximately 0.1μg/g), or (c) isotonic saline. A fourth group was handled, but not injected. On postnatal day 8 or 21, brain tissue was collected, fixed and sectioned. Free-floating sections were stained for estrogen receptor alpha using immunocytochemistry, and estrogen receptor alpha immunoreactive neurons were compared by age, treatment, and sex. To compare the temporal expression of estrogen receptor alpha an additional set of brains was collected from untreated males and females on the day of birth. The effects of oxytocin manipulations were age dependent, sexually dimorphic, and site-specific. While there were no significant treatment effects on postnatal day 8, by postnatal day 21 females that received oxytocin showed a significant increase in the number of cells expressing estrogen receptor alpha-immunoreactivity in the ventromedial nucleus of the hypothalamus. Treatment with oxytocin antagonist resulted in a significant decrease in estrogen receptor alpha-immunoreactivity in the medial preoptic area in postnatal day 21 females. While there were no significant effects in males, males treated with oxytocin antagonist trended toward a reduction in estrogen receptor alpha-immunoreactivity in the medial amygdala. The results indicate that oxytocin can have organizational effects on the expression of estrogen receptor alpha, that these effects are sexually dimorphic, and finally that during the preweaning period the development of estrogen receptor alpha is sexually dimorphic.