A flexible and wearable glucose sensor based on functional polymers with Soft-MEMS techniques
A flexible and wearable glucose sensor based on functional polymers with Soft-MEMS techniques
复制标题
DOI：
10.1016/j.bios.2006.05.006
复制
发表时间：
2006-10-15
影响因子：
12.6
通讯作者：
Mitsubayashi, Kohji
中科院分区：
文献类型：
--
作者：
Kudo, Hiroyuki;Sawada, Takanori;Mitsubayashi, Kohji
制备并测试了一种新型的功能聚合物葡萄糖传感器。该生物传感器利用疏水性聚二甲基硅氧烷（PDMS）和亲水性2-甲基丙烯酰氧乙基磷酸胆碱（MPC）与甲基丙烯酸二十二烷基酯（DMA）共聚的物理和化学功能。将葡萄糖氧化酶（GOD）固定在柔性过氧化氢电极（Pt工作电极和Ag/AgCl计数/参比电极）上构建葡萄糖传感器。利用微机电系统（MEMS）技术在这些功能聚合物上制备电极。该传感器具有新颖的柔性功能，可拉伸，将传感器的长度扩展到正常长度的120%后，释放后仍能正常工作。同时，对传感器的基本特性进行了评价。过氧化氢电极输出电流与过氧化氢浓度在0.20 ~ 2.50 mmol/l范围内呈线性相关，相关系数为0.998。然后用MPC聚合物将GOD固定在传感器表面。在本例中，葡萄糖传感器的电流输出与葡萄糖水平的相关范围为0.06-2.00 mmol/l，相关系数为0.997。校准范围包括正常人报告的泪液葡萄糖浓度（0.14 mmol/l）。(c) 2006 Elsevier B.V.版权所有
A novel biosensor for glucose measurement using functional polymers was fabricated and tested. The biosensor utilizes the physical and chemical functions of hydrophobic polydimethyl siloxane (PDMS) and hydrophilic 2-methacryloyloxyethyl phosphorylcholine (MPC) copolymerized with doclecyl methacrylate (DMA). The glucose sensor was constructed by immobilizing glucose oxidase (GOD) onto a flexible hydrogen peroxide electrode (Pt working electrode and Ag/AgCl counter/reference electrode). The electrodes were fabricated using microelectromechanical systems (MEMS) techniques onto those functional polymers. The sensor showed novel functions of flexibility and it was stretchable so that the sensor could normally work when it was released after expanding to 120% longer than that of normal length. Also, basic characteristics of the sensor were evaluated. The output current of the hydrogen peroxide electrode was linearly related to the hydrogen peroxide concentration in a range of 0.20-2.50 mmol/l, with a correlation coefficient of 0.998. GOD was then immobilized onto the surface of the sensor using MPC polymer. In this case, the current output of the glucose sensor related to the glucose level over a range of 0.06-2.00 mmol/l, with a correlation coefficient of 0.997. The calibration range includes the reported concentration of tear glucose in normal human subject (0.14 mmol/l). (c) 2006 Elsevier B.V. All rights reserved.