VibrioBase: A MALDI-TOF MS database for fast identification of Vibrio spp. that are potentially pathogenic in humans
VibrioBase: A MALDI-TOF MS database for fast identification of Vibrio spp. that are potentially pathogenic in humans
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DOI：
10.1016/j.syapm.2014.10.009
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发表时间：
2015-02-01
影响因子：
3.4
通讯作者：
Gerdts, Gunnar
中科院分区：
文献类型：
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作者：
Erler, Rene;Wichels, Antje;Gerdts, Gunnar
弧菌科的海洋细菌，特别是霍乱弧菌、副溶血性弧菌和创伤弧菌，被认为会引起人类的严重疾病。由于气候变化导致的温度升高，预计北方欧洲的弧菌丰度和感染率会更高，这反过来又需要环境监测计划来评估这种风险。我们建议，全细胞基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）分析是一个有前途的工具，快速和可靠的物种分类的环境菌株。由于参考数据库不包含足够的弧菌谱，我们在本研究中生成了vibrioBase数据库。质谱数据来自997个主要是环境菌株，并在这个新的数据库中存档。基于通过分析部分rpoB（RNA聚合酶β亚基）序列获得的物种分类，分配MALDI-TOF MS簇。在使用这两种方法的所有情况下，97%的菌株与物种特异性簇的联系是一致的，与市售数据库相比，扩展的VibrioBase产生了更特异的物种鉴定，具有更高的匹配分数。因此，我们免费提供了VibrioBase数据库，为潜在致病性弧菌的详细风险评估研究铺平了道路。从海洋环境中。(C)2014 Elsevier GmbH. All rights reserved.
Mesophilic marine bacteria of the family Vibrionaceae, specifically V. cholerae, V. parahaemolyticus and V. vulnificus, are considered to cause severe illness in humans. Due to climate-change-driven temperature increases, higher Vibrio abundances and infections are predicted for Northern Europe, which in turn necessitates environmental surveillance programs to evaluate this risk. We propose that whole-cell matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling is a promising tool for the fast and reliable species classification of environmental isolates. Because the reference database does not contain sufficient Vibrio spectra we generated the vibrioBase database in this study. Mass spectrometric data were generated from 997 largely environmental strains and filed in this new database. MALDI-TOF MS clusters were assigned based on the species classification obtained by analysis of partial rpoB (RNA polymerase beta-subunit) sequences. The affiliation of strains to species-specific clusters was consistent in 97% of all cases using both approaches, and the extended VibrioBase generated more specific species identifications with higher matching scores compared to the commercially available database. Therefore, we have made the VibrioBase database freely accessible, which paves the way for detailed risk assessment studies of potentially pathogenic Vibrio spp. from marine environments. (C) 2014 Elsevier GmbH. All rights reserved.