Discriminating symbiosis and immunity signals by receptor competition in rice
Discriminating symbiosis and immunity signals by receptor competition in rice
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通过水稻受体竞争区分共生和免疫信号
DOI：
10.1073/pnas.2023738118
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发表时间：
2021-04-20
影响因子：
11.1
通讯作者：
Wang, Ertao
中科院分区：
文献类型：
--
作者：
Zhang, Chi;He, Jiangman;Wang, Ertao
植物在自然界中遇到各种微生物，必须对共生或致病的微生物做出适当的反应。在水稻中，受体样激酶OsCERK1参与识别共生和免疫信号。然而，这些相反的信号是如何通过OsCERK1识别的仍然是未知的。在这里，我们发现受体竞争使水稻中的共生和免疫信号得以区分。一方面，共生受体OsMYR1及其短链壳寡糖配体抑制了OsCERK1和OsCEBiP之间复合体的形成，并抑制了OsCERK1对下游底物OsGEF1的磷酸化，从而降低了水稻对微生物相关分子模式的敏感性。的确，OsMYR1高表达系对稻瘟病菌更敏感，而Osmyr1突变体表现出更高的抗性。另一方面，OsCEBiP可以结合OsCERK1，从而阻止OsMYR1-OsCERK1异构体的形成。在感染的早期阶段，Oscebip突变体表现出更高的菌根定殖率。我们的结果表明，OsMYR1和OsCEBiP受体竞争OsCERK1来决定共生和免疫信号的结果。
Plants encounter various microbes in nature and must respond appropriately to symbiotic or pathogenic ones. In rice, the receptor-like kinase OsCERK1 is involved in recognizing both symbiotic and immune signals. However, how these opposing signals are discerned via OsCERK1 remains unknown. Here, we found that receptor competition enables the discrimination of symbiosis and immunity signals in rice. On the one hand, the symbiotic receptor OsMYR1 and its short-length chitooligosaccharide ligand inhibit complex formation between OsCERK1 and OsCEBiP and suppress OsCERK1 phosphorylating the downstream substrate OsGEF1, which reduces the sensitivity of rice to microbe-associated molecular patterns. Indeed, OsMYR1 overexpression lines are more susceptible to the fungal pathogen Magnaporthe oryzae, whereas Osmyr1 mutants show higher resistance. On the other hand, OsCEBiP can bind OsCERK1 and thus block OsMYR1-OsCERK1 heteromer formation. Consistently, the Oscebip mutant displayed a higher rate of mycorrhizal colonization at early stages of infection. Our results indicate that OsMYR1 and OsCEBiP receptors compete for OsCERK1 to determine the outcome of symbiosis and immunity signals.