Isolation, characterization, and chromosome assignment of mouse N-ras gene from carcinogen-induced thymic lymphoma.
Isolation, characterization, and chromosome assignment of mouse N-ras gene from carcinogen-induced thymic lymphoma.
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致癌物诱导的胸腺淋巴瘤小鼠 N-ras 基因的分离、表征和染色体分配。
DOI：
10.1126/science.6089339
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Pellicer,A
中科院分区：
文献类型：
--
作者：
Guerrero,I;Villasante,A;D'Eustachio,P;Pellicer,A
用致癌物N-甲基亚硝基脲治疗小鼠会导致胸腺淋巴瘤的发生，并经常涉及N-rasoncogene。从这些淋巴瘤之一中分离出激活的小鼠N-rasgene，并通过与限制性消化相结合的转化，制备了该基因的图谱并确定了其大致边界。通过体细胞杂交，发现正常的 N-rasgene 与 therasgene 家族的其他成员无关。
Treatment of mice with the carcinogenN-methylnitrosourea results in the development of thymic lymphomas with frequent involvement of the N-rasoncogene. The activated mouse N-rasgene was isolated from one of these lymphomas and, by transformation in concert with restriction digestion, a map of the gene was prepared and its approximate boundaries were determined. By means of somatic cell hybrids the normal N-rasgene was found to be unlinked to other members of therasgene family.