Enhanced in vivo sensitivity to interferon with in vitro resistant B16 tumor cells in mice.
Enhanced in vivo sensitivity to interferon with in vitro resistant B16 tumor cells in mice.
复制标题
增强小鼠体外抗性 B16 肿瘤细胞对干扰素的体内敏感性。
DOI：
10.1007/bf01533379
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
FleischmannJr,WR
中科院分区：
文献类型：
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作者：
Fleischmann,CM;Stanton,GJ;FleischmannJr,WR
当小鼠 B16 黑色素瘤细胞在体外暴露于干扰素时，它们会迅速对干扰素 α (IFNα) 和干扰素 β (IFNβ) 的抗增殖作用产生抵抗力。这种耐药性的特点是非遗传性和剂量依赖性，并且不会改变 IFN 诱导的其他作用，例如抗病毒作用和 IFN 处理细胞中 2',5'-寡腺苷酸合成酶活性的升高。对这些干扰素耐药细胞的研究已扩展到体内肿瘤模型。如果体内出现耐药性，则不会对接受 IFN 治疗的小鼠的生存产生不利影响。此外，接种体外具有抗性的 B16 细胞（B16αrescells）的 IFN 治疗小鼠的存活时间明显长于接种体外敏感的 B16 细胞的 IFN 治疗小鼠。经 IFN 治疗的 B16αres 接种小鼠的治愈率也明显较高。携带B16αrescell肿瘤的小鼠的存活时间延长似乎并不是由B16αrescell生长速度较慢引起的，因为使用高十倍的B16αrescell接种物和低十倍的B16细胞接种物进行的实验没有显示存活模式有任何变化。很明显，体外耐药的 B16αrescells 对体内 IFN 诱导的抗肿瘤作用比体外敏感的 B16 细胞更敏感。
Mouse B16 melanoma cells rapidly develop resistance to the antiproliferative effects of interferon α (IFNα) and interferon β (IFNβ) when they are exposed to the interferons in vitro. This resistance was characterized to be non-genetic and dose-dependent, and does not alter other IFN-induced effects such as antiviral effects and elevation of 2′,5′-oligoadenylate synthetase activity in IFN-treated cells. The study of these IFN-resistant cells has been extended to an in vivo tumor model. Resistance, if it occurred in vivo, did not adversely affect the survival of IFN-treated mice. Further, IFN-treated mice inoculated with B16 cells that were resistant in vitro (B16αrescells) survived significantly longer than IFN-treated mice inoculated with B16 cells that were sensitive in vitro. The IFN-treated B16αres-inoculated mice had a significantly higher cure rate as well. The prolonged survival of the mice bearing B16αrescell tumors did not seem to be caused by the slower growth rate of the B16αrescells, since experiments performed with a tenfold higher B16αrescell inoculum and a tenfold lower B16 cell inoculum did not show any change in the survival pattern. It is clear that in vitro resistant B16αrescells are more sensitive to antitumor effects induced by IFN in vivo than in vitro sensitive B16 cells.